Microalgae-biochar compound biological fertilizer and preparation method thereof
A compound bio-fertilizer and bio-char technology, applied to organic fertilizers, fertilization devices, fertilizer mixtures, etc., can solve problems such as increasing soil fertility, low mineral nutrient content, and differences in bio-char mineral nutrient content, so as to improve soil fertility and planting Effects on plant yield, increase in soil available phosphorus content, and increase in organic matter content
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Embodiment 1
[0031] Example 1 Microalgae-biochar-based fertilizer supplemented with crop waste to improve sandy / desertified soil
[0032] Microtheca was isolated and purified from sandy soil crusts by BG11 nitrogen-deficient medium plate method several times. In a 60L photobioreactor (1%CO 2 , LED light intensity 60μmol photon m -2 ·s -1 , 25°C) for 7 days of scale-up culture with the exclusive medium prepared above to screen and cultivate microalgal species with high nitrogen-fixing capacity and high carbon-fixing capacity. After the biomass reaches 0.9g / L, the microalgae are enriched by gravity sedimentation and scaled up. Then, use the new thin layer (RW) drying technology to collect active microalgae, and prepare compound biofertilizer according to the mass ratio of microalgae:biochar:crop waste equal to 15:35:50.
[0033] After applying the compound biological fertilizer, the following effects are achieved:
[0034] 1. It can effectively improve the aggregate structure of sandy s...
Embodiment 2
[0039] Example 2 Microalgae-biochar base fertilizer supplemented with organic fertilizer and quick-acting fertilizer to improve compacted or barren soil
[0040]Nostoc was isolated and purified several times from hardened soil crusts by BG11 nitrogen-deficient medium plate method. In a 60L photobioreactor (2%CO 2 , LED light intensity 100μmol photon m -2 ·s -1 , 25°C), use the above-mentioned special medium prepared above which can represent the floor junction or barren soil environment to carry out amplified culture for 7 days to screen and cultivate microalgal species with high nitrogen fixation capacity and high carbon fixation capacity. After the biomass reached 1.2g / L, the microalgae were enriched by filtration and carried out on a large scale, and then the microalgae were treated overnight by vacuum freeze-drying technology to keep the microalgae active. Finally, active microalgae are collected, and compound biofertilizer is prepared according to the mass ratio of mic...
Embodiment 3
[0048] Example 3 Microalgae-biochar-based fertilizer supplemented with alkaline slag to improve acidified soil
[0049] Anabaena was isolated and purified from acidified soil crusts by BG11 nitrogen-deficient medium plate method. In a 60L photobioreactor (1.5%CO 2 , LED light intensity 60μmol photon m -2 ·s -1 , 25°C), use the above-mentioned special culture medium that can represent the acidified soil environment of the territory to carry out amplified culture for 7 days to screen and cultivate microalgal species with high nitrogen fixation capacity and high carbon fixation capacity. After the biomass reached 1.0g / L, the microalgae were enriched by centrifugation at 4000×g, and then cultured on a large scale. Then, spray drying is performed to keep the microalgae alive. Finally, collect microalgae, according to microalgae: biochar: alkaline slag (pH value 9-12, the CaCl that contains mass ratio 35%) 2 , 40% CaCO 3 , 25% CaSO 4 ): The mass ratio of crop waste equal to 1...
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