Citrinin compound penicitrinol N derived from penicillium citrinum as well as preparation method and application thereof

A compound, the technology of Penicillium citrinum, is applied in the field of citrinin compounds to achieve the effect of significant anti-tumor activity

Inactive Publication Date: 2015-03-25
FUZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The study found that the alkaloid compound shown has anti-tumor activity, and there is no report on the inhi

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  • Citrinin compound penicitrinol N derived from penicillium citrinum as well as preparation method and application thereof
  • Citrinin compound penicitrinol N derived from penicillium citrinum as well as preparation method and application thereof
  • Citrinin compound penicitrinol N derived from penicillium citrinum as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1 Fermentation production and separation and purification of the compound

[0019] 1 Fermentation production

[0020] Fermentation culture of producing bacteria: Take Penicillium citrinum ( Penicillium citrinum ) IBPT-5 (has been deposited in the China Type Culture Collection on December 25, 2013, address: Wuhan University, Wuhan, deposit number: CCTCC NO: M 2013713) appropriate amount, inoculated on the PDA solid slant medium at 28 Cultivate in an incubator at ℃ for 4 days.

[0021] Take the penicillium citrinum ( Penicillium citrinum ) An appropriate amount of IBPT-5 is inoculated into 400mL culture solution [Composition of culture solution (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5, MgSO 4 0.3, NaCl 30.0 constant volume] 1000mL Erlenmeyer flask, 28 ℃ static culture for 30 days, obtain mycelium and fermentation broth.

[0022] 2 Obtaining extract

[0023] Use gauze to separate the mycelium from the f...

Example Embodiment

[0029] Example 2 In vitro anti-tumor activity test

[0030] 1 Experimental samples and experimental methods

[0031] Preparation of the test sample solution The test sample is the pure compound separated and refined in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample and prepare a solution of the required concentration with methanol for activity measurement.

[0032] Tumor cell lines were used for the subculture of cell lines and cells. Tumor cells were subcultured in DMEM medium containing 10% FBS at 37°C in an incubator filled with 5% carbon dioxide.

[0033] Cell proliferation inhibitory activity test method

[0034] Tetrazolium salt (MTT) method Take tumor cells in logarithmic growth phase and adjust the cell density to 2×10 per ml 5 Cells were seeded in a 96-well cell culture plate at 200 microliters per well, and 5% CO was injected at 37℃ 2 Incubate in the incubator for 4 hours. Add 2 microliters of sample solution or blank solution to each ...

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Abstract

The invention relates to a citrinin compound penicitrinol N derived from penicillium citrinum as well as a preparation method and application thereof. The compound has the function of inhibiting tumor cell proliferation and has antitumor activity. A structural formula of the compound is shown in the specification. The preparation method is characterized by obtaining a fermentation product through fermentation culture of penicillium citrinum IBPT-5 and then separating and purifying the compound from the fermentation product. Experiments prove that the compound has better antitumor activity for a human cervical cancer cell line hela and can be used for preparing cell proliferation inhibition drugs or antitumor drugs for antitumor studies.

Description

Technical field [0001] The invention relates to a citrinin compound penicitrinol N derived from Penicillium citrinum and its preparation method and application. Background technique [0002] Fungi are relatively high among microorganisms. Fungal natural products are an important source of activity diversity and structural diversity, and many chemical drugs with good therapeutic effects have been developed. For example, antibiotic penicillin (penicillin) series, immunosuppressant cyclosporin A (cyclosporin A), lipid-lowering drugs mevastatin (mevastatin), lovastatin (lovastatin) and antifungal drug griseofulvin (griseofulvin), etc. All these show that fungi are an important treasure house of natural medicine. Citrinin, a well-known secondary metabolite of polyketide fungi, was first isolated from the secondary metabolite of a strain of Penicillium citrinum. In recent years, studies have found that citrinin compounds exhibit gratifying biological activities such as anti-tumor, a...

Claims

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Application Information

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IPC IPC(8): C07D493/06C12P17/18A61K31/352A61P35/00C12R1/80
CPCC07D493/06C12P17/181
Inventor 陈立毕延雪周彤张其清
Owner FUZHOU UNIVERSITY
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