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Anaerobic blood culture medium, culture bottle and preparation method of culture bottle

A culture medium and blood culture technology, applied in the field of medicinal chemistry, can solve the problems of low positive detection rate, high cost, complicated production, etc., and achieve the effect of promoting bacterial growth, significant progress, and highlighting substantive characteristics

Active Publication Date: 2015-03-25
CHENGDU RICH SCI IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a kind of anaerobic blood medium, a culture bottle synthesized from this culture medium and a culture method of the culture bottle. The positive detection rate is low, the production is complicated, and the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Anaerobic blood culture medium A component is: caseinase hydrolyzate 10g, yeast extract powder 5g, soybean peptone 3g-, glucose 3g, disodium hydrogen phosphate 2g, potassium dihydrogen phosphate 1g, sodium citrate 2g, reducing Iron powder 1g, agar powder 2g, sodium bicarbonate 1g, sodium thioglycolate 1g;

[0022] Component B is: Sodium polyanethole sulfonate (SPS) 0.1g, p-aminobenzoic acid (PABA) 0.03g, glucose oxidase (gOD) 5.0mg, catalase (CAT) 5.0mg, hemin 20.0mg, oxidative coenzyme Ⅰ (NAD) 20.0mg.

[0023] The oxygen content of the anaerobic blood culture bottle prepared in Example 1 is 200-400ppm, which is more than 50% lower than the oxygen content of the traditional anaerobic blood culture bottle (400-1000ppm on the market), and is stable, and the positive detection rate is higher than The traditional anaerobic blood culture bottle is more than 20% higher.

Embodiment 2

[0025] Anaerobic blood medium A component is: 20g of caseinase hydrolyzate, 15g of yeast extract powder, 10g of soybean peptone, 20g of glucose, 10g of disodium hydrogen phosphate, 4g of potassium dihydrogen phosphate, 5g of sodium citrate, reduced iron powder 4g, agar powder 5g, sodium bicarbonate 4g, sodium thioglycolate 2.5g;

[0026] Component B is sodium polyanethole sulfonate (SPS) 0.4g, p-aminobenzoic acid (PABA) 0.12g, glucose oxidase (gOD) 10.0g, catalase (CAT) 10.0g, hemin 50.0g mg, oxidative coenzyme Ⅰ (NAD) 50.0mg.

[0027] The oxygen content of the anaerobic blood culture bottle prepared in Example 2 is 200-400ppm, which is more than 50% lower than the oxygen content of the traditional anaerobic blood culture bottle (400-1000ppm on the market), and is stable, and the positive detection rate is higher than The traditional anaerobic blood culture bottle is more than 20% higher.

Embodiment 3

[0029] Anaerobic blood medium A component is: caseinase hydrolyzate 13g yeast extract powder 8g, soybean peptone 5g, glucose 7g, disodium hydrogen phosphate 4g, potassium dihydrogen phosphate 2g, sodium citrate 3g, reduced iron powder 2g, agar powder 3g, sodium bicarbonate 2g, sodium thioglycolate 1.5g;

[0030] Component B is: Sodium polyanethole sulfonate (SPS) 0.2g, p-aminobenzoic acid (PABA) 0.06g, glucose oxidase (gOD) 20.0mg, catalase (CAT) 20.0mg, hemin 30.0mg, oxidative coenzyme Ⅰ (NAD) 30.0mg.

[0031] The oxygen content of the anaerobic blood culture bottle prepared in Example 3 is 200-400ppm, which is more than 50% lower than the oxygen content of the traditional anaerobic blood culture bottle (400-1000ppm on the market), and is stable, and the positive detection rate is higher than The traditional anaerobic blood culture bottle is more than 20% higher.

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Abstract

The invention discloses an anaerobic blood culture medium, a culture bottle prepared from the culture medium and a preparation method of the culture bottle. Two matters of glucose oxidase and peroxidase are added in the culture medium disclosed by the invention, and the matters can remove oxygen in the culture medium and hydrogen peroxide and other cytotoxic substances which are toxic and harmful to bacteria and are generated in a metabolic process, so that the problems in traditional methods of low positive detection rate, complicated production and high cost are solved.

Description

technical field [0001] The invention relates to the field of medicinal chemistry, in particular to an anaerobic blood culture medium, a culture bottle synthesized from the culture medium and a culture method of the culture bottle. Background technique [0002] The traditional anaerobic blood culture bottle removes oxygen from the blood culture bottle by gas replacement and adding a reducing agent to the medium, reduces the redox potential, and creates a good growth environment for anaerobic bacteria. The oxygen content of the blood culture bottles produced by the traditional method is still high, and some of the added reducing agents have different degrees of inhibition on anaerobic bacteria, resulting in a low positive detection rate and high production costs. In this method, glucose oxidase and catalase are added to the medium to remove oxygen in the medium and metabolites such as hydrogen peroxide, which are toxic to bacteria, produced during the metabolic process. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/24C12N1/20
CPCC12M23/08C12N1/20
Inventor 张力
Owner CHENGDU RICH SCI IND
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