An anti-interference one-component enzyme substrate chromogenic reagent

Abstract

The invention discloses an anti-interference one-component enzyme substrate color developing reagent. Each 1,000 mL of color developing liquid comprises the following components: 7-10 g of citric acid, 16-20 g of disodium hydrogen phosphate, 0.1-0.5 g of sodium perborate, 20-30 ml of glycerin, 5-40 mg of an enzyme substrate and 10-30 mL of ethanol; the pH value of the anti-interference one-component enzyme substrate color developing reagent is 4.0-6.0. By adjusting the pH value of the anti-interference one-component enzyme substrate color developing reagent to be 4.0-6.0, the anti-interference one-component enzyme substrate color developing reagent is relatively good in stability when being used for the color developing period in an enzyme-linked immunosorbent assay reaction, and has a relatively small color developing background; moreover, due to the one-component color developing liquid, the step of mixing liquid A with liquid B during use of the enzyme substrate color developing reagent is avoided, the operating time is shortened, the efficiency is improved, the concentration change of the effective color developing components of the enzyme substrate color developing reagent is avoided, and then the sensitivity of the enzyme substrate color developing reagent is improved.

Description

technical field [0001] The invention relates to the field of enzyme substrate chromogenic reagents, in particular to an anti-interference one-component enzyme substrate chromogenic reagent. Background technique [0002] The enzyme substrate is TMB, which is used for ELISA reaction. The substrate produces a soluble light blue end product that can be read on a spectrophotometer at 370 or 635 nm. It is suitable for quantitative and qualitative analysis in the chromogenic stage of ELISA. The principle of the test is: when the enzyme labeled on the antibody or antigen reacts with the enzyme substrate, hydrogen peroxide, etc. in the chromogenic solution, the solution changes from colorless to yellow after adding the stop solution, and the depth of yellow reflects the combination of antigen and antibody in the ELISA system How much, so as to judge the amount of a certain antigen or antibody. Measure the absorbance value with a microplate reader to judge the degree of immune reac...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide an anti-interference one-component enzyme substrate chromogenic reagent to overcome the problems of high color background, poor stability and low sensitivity of existing chromogenic liquids

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