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A high-throughput capture method for imaging flow cytometry

A technology of flow cytometer and shooting method, which is applied in image communication, parts of color TV, parts of TV system, etc., can solve the problems of low shooting efficiency, low luminous flux, missed shots, etc. The effect of increasing the luminous flux

Inactive Publication Date: 2017-07-25
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to propose a high-throughput shooting method for imaging flow cytometers to solve the problems of low shooting efficiency, low luminous flux and missing shots in the prior art

Method used

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  • A high-throughput capture method for imaging flow cytometry
  • A high-throughput capture method for imaging flow cytometry

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Experimental program
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Embodiment 1

[0031] See attached figure 2 , the device used to realize the high-throughput shooting method for imaging flow cytometer of the present invention includes micropipe 2, transmission device 3, camera A5, camera B6 and computer 7; said camera A5 and camera B6 respectively The tracking and shooting of the cells in the micro-channel 2 is realized by driving the transmission device 3 , and the movement of the transmission device 3 is controlled by a computer 7 .

[0032] A high-throughput shooting method for imaging flow cytometer of the present invention comprises the following steps:

[0033] Step 1: Cell 1 moves from top to bottom from microchannel 2, the flow rate of cell 1 is 2V, and the cells in microchannel 2 are sequentially numbered as n, n+1, n+2...n+m from bottom to top;

[0034] Step 2: When the n+1th cell enters the field of view of the camera A5, the camera A5 starts to work and takes pictures of the n+1th cell, and the camera A5 drives the n+1th cell by △V through t...

Embodiment 2

[0040] Cell 1 flows from top to bottom along the microchannel 2, we divide two adjacent cells 1 into a group, see attached figure 2, draw four groups of cells respectively, numbered as group 1 to group 4 from bottom to top according to the flow order of cell 1. And cell 1 is numbered from bottom to top, respectively: 1 group n, n+1, 2 groups n+2, n+3, 3 groups n+4, n+5 and 4 groups n+6, n+7 The cells in group 1 flow through camera A5 first, and then through camera B6. When cell n+1 in group 1 enters the field of view of camera A5, camera A5 starts to work, and camera A5 follows the cells under the action of transmission device 3. The flow of n+1 moves in the same direction to complete the exposure imaging of cell n+1 in the field of view of camera A for △t time, and then camera A5 quickly resets, waiting for the next group of cell 1 to be photographed. Then cell n of group 1 enters the field of view of camera B6, and cell n+3 of group 2 enters the field of view of camera A5....

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Abstract

A high-throughput shooting method for imaging flow cytometer belongs to the field of imaging technology, and aims to solve the problems of low shooting efficiency, low luminous flux and missing shots in the prior art. The invention includes the following steps: firstly, the cells move from the top to the bottom of the microchannel, and when the first even-numbered cell enters the field of view of the camera A, the camera A drives the transmission device to track and shoot the first even-numbered cell, and the camera After A completes the shooting, it resets to the initial position through the transmission device, shoots the next even-numbered cell, and transmits the collected image to the computer; when the first odd-numbered cell enters the field of view of camera B, camera B passes through The transmission device drives the first odd-numbered cell to be tracked and photographed, and the camera B is reset to the initial position through the transmission device after shooting, and the next odd-numbered cell is photographed, and the collected image is transmitted to the computer; repeat the above steps until All cell shots are done.

Description

technical field [0001] The invention belongs to the technical field of imaging, and in particular relates to a high-throughput shooting method for imaging flow cytometers. Background technique [0002] Cells are living bodies that make up various tissues and organs, and the health of cells directly affects human health. Microscopic detection of cells is an effective way to judge human health and discover potential tissue lesions, which requires clear and stable imaging of cells. [0003] See attached figure 1 , the traditional method for cell detection is to let the cells pass through the field of view of the lens sequentially, and take pictures of the cells 1 sequentially. The device for realizing the above method includes a microchannel 2, a camera 4 and a computer 7. This method has only one lens, and the cells in the microchannel The flow velocity is V, and the cell 1 passes through the lens of the camera 4 sequentially. The integration time from entering the field of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H04N5/232H04N5/235
Inventor 张红鑫卢振武孙强赵建卜和阳
Owner CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI