Applications of dihydrochalcone and dihydrochalcone glucoside compound as active ingredients used for whitening skin and improving dark and gloomy skin
A technology of dihydrochalcone and active ingredients, which is applied in the field of application of dihydrochalcone and its glycosides as active ingredients for whitening and improving dull skin, and can solve the problem of single ingredients, no prevention or treatment of melanin, and improvement of skin. Issues such as reports in food, beverages, cosmetics, or pharmaceuticals
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0034] Tyrosinase Inhibition Test
[0035] 1. Determination of tyrosinase activity
[0036] 15 μL 5% DMSO solution, 60 μL 0.25 mmol / L L-Dopa, 195 μL pH 6.8 phosphate buffer solution, shake well. After incubation at 37°C for 5 min, 30 μL of tyrosinase solution was added and mixed immediately. Measure the absorbance value at 475 nm with a multi-functional microplate reader at a constant temperature of 37°C, make three parallel samples for each sample, and use the system without enzyme as a blank to draw a straight line of the increase of absorbance with time, from the slope of the straight line Enzyme activity A 0 .
[0037] 2. Determination of tyrosinase enzyme activity under the action of samples
[0038] 15 μL samples of different concentrations (5% DMSO), 60 μL 0.25 mmol / L L-Dopa, 195 μL pH 6.8 phosphate buffer solution, shake well. After incubating at 37°C for 5 minutes, add 30 μL of tyrosinase solution and mix immediately. Measure the absorbance value at 475 nm with ...
Embodiment 2
[0048] Inhibition of melanin production in B16 melanoma cells
[0049] Using NaOH cracking method. The day before the test, the cells were subcultured to make the confluence 30%-50%, and the DMEM-high glucose medium was used for the test. For example, a 6-well plate is used, the diameter of each well is 34.8mm, and the growth area is 9.5cm 2 . The volume of culture medium is 2000 μL. Add samples for processing the next day, when the confluence reaches 80%-90%, the cell volume is 1×10 6 / hole. When the eyes see obvious color changes in the medium, and the microscope observes black precipitates in the cells. After about 48 hours, the cells were collected for detection. Harvest 1×10 6 Wash the cells twice with PBS, add 200 μL redistilled water to suspend the precipitate, add 1 mL of ethanol:ether (1:1) mixture, let stand at room temperature for 20 min, centrifuge at 3000 r / min for 5 min, and add the precipitate 1 mol / L NaOH 1 mL (containing 10% DMSO), incubate in a water ...
Embodiment 4
[0055] Preparation of gel-like peel-off mask
[0056] In 65g of pure water, add 5g of carboxymethylcellulose and 10g of polyvinyl alcohol, dissolve while heating, add 0.1g of hesperidin dihydrochalcone and 19g of ethanol at the same time, add a small amount of citrus essence, Makes into a gel-like peel-off mask.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com