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Preparation method and application of capsaicin-collagen sponge

A collagen sponge and collagen technology, applied in the field of preparation of capsaicin-collagen sponge, can solve the problems of skin irritation, short half-life and the like, and achieve the effects of reducing irritation, good biocompatibility, and increasing drug efficacy

Active Publication Date: 2015-04-08
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, capsaicin has a high degree of hepatic first-pass effect, and its half-life is relatively short, so capsaicin is suitable for topical transdermal administration in the form of creams and patches. skin irritation

Method used

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  • Preparation method and application of capsaicin-collagen sponge
  • Preparation method and application of capsaicin-collagen sponge
  • Preparation method and application of capsaicin-collagen sponge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation method of the capsaicin-collagen sponge that the present embodiment provides, it may further comprise the steps:

[0043] Step 1: mechanically remove the fascia, muscle and fat from bovine Achilles tendon rich in collagen, then fully pulverize it, wash it with pure water after pulverization, and obtain collagen powder;

[0044] Step 2, dissolving the collagen powder obtained in Step 1 in a dilute acid solution, adding pepsin with an activity of 1:10000 to the dilute acid solution, and then stirring and extracting at 4°C for 36 hours to obtain an extract; In an embodiment, the pH value of the acetic acid solution is 2.5. In this embodiment, the weight ratio of collagen powder to pepsin is 50:1.

[0045]Step 3, centrifuging the extract obtained in step 2 and collecting the supernatant;

[0046] Step 4, adding a concentration of 10mol / L sodium hydroxide solution to the supernatant obtained in step 3 is neutral to the pH of the supernatant, and collagen is...

Embodiment 2

[0052] The preparation method of the capsaicin-collagen sponge that the present embodiment provides, it may further comprise the steps:

[0053] Step 1: Mechanically remove fascia, muscle and fat from pig Achilles tendon rich in collagen, then fully pulverize it, wash it with pure water after pulverization, and obtain collagen powder;

[0054] Step 2, dissolving the collagen powder obtained in Step 1 in a dilute acid solution, and adding pepsin with an activity of 1:10000 to the dilute acid solution, and then stirring and extracting at 3°C ​​for 48 hours to obtain an extract; In the embodiment, the pH value of the acetic acid solution is 2. In this embodiment, the weight ratio of collagen powder to pepsin is 40:0.5.

[0055] Step 3, centrifuging the extract obtained in step 2 and collecting the supernatant;

[0056] Step 4, adding concentration to the supernatant obtained in step 3 is 8mol / L sodium hydroxide solution until the pH of the supernatant is neutral, collagen is pr...

Embodiment 3

[0062] The preparation method of the capsaicin-collagen sponge that the present embodiment provides, it may further comprise the steps:

[0063] Step 1: Mechanically remove the fascia, muscle and fat from the sheep Achilles tendon rich in collagen, then fully pulverize it, wash it with pure water after pulverization, and obtain collagen powder;

[0064] Step 2, dissolving the collagen powder obtained in Step 1 in a dilute acid solution, and adding pepsin with an activity of 1:10000 to the dilute acid solution, and then stirring and extracting at 5°C for 24 hours to obtain an extract; In the embodiment, the pH value of the acetic acid solution is 3. In this embodiment, the weight ratio of collagen powder to pepsin is 60:1.5.

[0065] Step 3, centrifuging the extract obtained in step 2 and collecting the supernatant;

[0066] Step 4, adding a concentration of 12mol / L sodium hydroxide solution to the supernatant obtained in step 3 is neutral to the pH of the supernatant, and co...

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Abstract

The invention relates to the technical field of external medicines for hypertrophic scars, and particularly relates to a preparation method and an application of capsaicin-collagen sponge. The preparation method comprises the following steps: 1 preparing collagen powder from tendo calcaneus; 2 extracting the collagen powder with pepsin, so as to obtain an extract liquid; 3 centrifugally collecting supernate; 4 separating out collagen by adding sodium hydroxide, and washing, so as to prepare high-purity collagen; 5 redissolving the collagen with ethylic acid; and 6 evenly mixing the collagen liquid, glycerinum and capsaicin, and then carrying out freeze-drying, so as to obtain the capsaicin-collagen sponge. The capsaicin-collagen sponge prepared by the method is applied to tissue trauma repair and inhibition of the hypertrophic scars in the tissue trauma repair process, and has the advantage of good percutaneous permeability; the drug liver first-pass effect can be avoided; and the thrill to the skin can be reduced.

Description

Technical field [0001] The technology field of external drugs involving hyperplasia scars, especially the preparation methods and applications of chiliine-collagen sponge. Background technique [0002] A pathological scar of hyperplasia is a product of soft skin tissue after being traumatized.It often occurs after surgery, trauma and burns, which is the complication of damage to the skin to repair in March.Proliferative scars not only affect the aesthetics, but also cause itching, pain and other symptoms, and even cause severe dysfunction.In existing technologies, clinically common topical drugs include silicone membrane, silicon gel, ointment, etc., but the effect of these external drug carriers is not ideal, mainly because these drugs are not easy to enter the scar tissue.And these external drugs generally contain toxic chemical cross -linking agents, so that the safety and biocompatibility of these external drugs are not good.In addition, due to the special pathological struct...

Claims

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Application Information

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IPC IPC(8): A61K9/00A61K31/165A61K47/42A61P17/02
Inventor 彭新生周艳芳郭春连陈红英陈宇欢李洁媚黎思彤何大华戴忠周艳星王勤
Owner GUANGDONG MEDICAL UNIV
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