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Hybridoma cell, pcv2 monoclonal antibody and application thereof

A monoclonal antibody and hybridoma cell technology, applied in the direction of antibodies, antiviral agents, antiviral immunoglobulin, etc., can solve the problems of specificity judgment and sensitivity limitation, misjudgment of positive signal cells, etc., and achieve high sensitivity and high specific effect

Inactive Publication Date: 2017-10-03
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

[0005] At present, in the immunohistochemical detection of PCV2 positive cells, self-made polyclonal antibodies of porcine or mouse immune serum are generally used. Although porcine PCV2 positive cells can be judged, the specificity judgment and sensitivity are limited, which inevitably causes researchers to be suspicious of positive signals. Misjudgment of cells, therefore, it is still necessary to improve the existing PCV2 antibody to improve the specificity and sensitivity of the antibody, thereby improving the accuracy of judgment

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  • Hybridoma cell, pcv2 monoclonal antibody and application thereof
  • Hybridoma cell, pcv2 monoclonal antibody and application thereof
  • Hybridoma cell, pcv2 monoclonal antibody and application thereof

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Embodiment Construction

[0024] It should be noted that, in the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and examples.

[0025] As mentioned in the background technology section, in the process of detecting PCV2 virus infection, the polyclonal antibody or monoclonal antibody prepared based on PCV2 protein in the prior art is relatively poor in specificity and sensitivity, which easily leads to misjudgment Defects. In order to improve the above-mentioned situation, the inventor of the present invention has carried out a large amount of experiments and research to PCV2, and content of the present invention is made on the basis of following research result exactly:

[0026] The present invention creatively adopts self-developed PCV2 virus-like particles (PCV2 VLPs) as immunogen to carry out follow-up research work....

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Abstract

The invention provides a hybridoma cell, PCV2 monoclonal antibody and application thereof. The present invention creatively adopts PCV2 virus-like particles as immunogens, since PCV2 virus-like particles are an empty shell structure assembled from proteins closer to normal PCV2 viruses, they can be presented to immune cells through the same way as normal virus infection Inducing the immune system to produce an immune protection response, so that the monoclonal antibody secreted by the hybridoma cells of the present invention has higher specificity and sensitivity than the PCV2 antigen protein monoclonal antibody.

Description

technical field [0001] The invention relates to the field of immune detection, in particular to a hybridoma cell, a PCV2 monoclonal antibody and applications thereof. Background technique [0002] Porcine circovirus is a single-stranded circular DNA virus belonging to the genus Circovirus in the family Circoviridae. The virion is a non-enveloped 20-hedron symmetric structure with a diameter of about 17nm. The genome fragment size is 1767bp or 1768bp, mainly including three open reading frames (ORFs). ORF1 encodes replication-related proteins, and the Cap protein encoded by ORF2 is the virus The main immunogenic protein, ORF3, is involved in virus infection and pathogenesis. Porcine circovirus is currently divided into two serotypes: PCV1 and PCV2. PCV2 can be divided into three genotypes, PCV2a, PCV2b and PCV2c. During the epidemic process, the PCV2 genotype changes from PCV2a to PCV2b, and the virulence also shows a trend of enhancement. PCV2c has only been reported in D...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/20C07K16/08G01N33/577G01N33/569A61K39/42A61P31/20B01D15/38C12M1/00
Inventor 王乃东杨毅邓治邦王爱兵朱哲蔡杰雷昕诺张颜湛洋
Owner HUNAN AGRICULTURAL UNIV
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