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Single reagent serum triglyceride detection reagent with strong stability

A detection reagent and technology with strong stability, applied in the field of single-reagent serum triglyceride detection reagents, can solve the problems of poor stability and the reagents are easily accepted by environmental pollution, achieve protection stability, improve anti-interference ability and stability , the effect of improving stability

Active Publication Date: 2015-04-08
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally, there are more dual-reagent TG detection reagents on the market, while single-reagent TG detection reagents are not accepted by the market due to their poor stability and the reagents are extremely susceptible to environmental pollution.

Method used

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  • Single reagent serum triglyceride detection reagent with strong stability
  • Single reagent serum triglyceride detection reagent with strong stability
  • Single reagent serum triglyceride detection reagent with strong stability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A single-reagent serum triglyceride detection reagent with strong stability, the reagent includes: 50mmol / L 4-hydroxyethylpiperazineethanesulfonic acid-sodium glutamate buffer solution (pH=7.4), 1g / L bovine Serum albumin, 0.1g / L triethanolamine lauryl sulfate, 0.1g / L sodium perbenzoate, 1g / L coenzyme FAD, 0.1g / L polyvinyl alcohol AH-26, 10mmol / L ethylenediaminetetraacetic acid Sodium, 3.5mmol / L sodium cholate, 17.5mo / L magnesium sulfate heptahydrate, 1mmol / L 4-aminoantipyrine, 3.5mmol / L N, N-dimethylaniline, 0.1g / L TritonX-100, 1KU / L lipoprotein lipase, 0.25KU / L glycerol kinase, 1KU / L peroxidase, 1KU / L glycerol-3-phosphate oxidase, 0.1g / L sodium azide.

[0022] The above-mentioned components were successively dissolved in 1 L of distilled water to complete the preparation.

Embodiment 2

[0024] A single-reagent serum triglyceride detection reagent with strong stability, the reagent includes: 60mmol / L 4-hydroxyethylpiperazineethanesulfonic acid-sodium glutamate buffer solution (pH=7.4), 5g / L bovine Serum albumin, 1g / L triethanolamine lauryl sulfate, 1g / L sodium perbenzoate, 2g / L coenzyme FAD, 1g / L polyvinyl alcohol AH-26, 100mmol / L disodium edetate, 5mmol / L sodium cholate, 20.5mo / Ll magnesium sulfate heptahydrate, 3mmol / L 4-aminoantipyrine, 5mmol / L N,N-dimethylaniline, 1g / L TritonX-100, 3KU / L lipoprotein fat Enzyme, 500KU / L glycerol kinase, 3KU / L peroxidase, 3KU / L glycerol-3-phosphate oxidase, 1g / L sodium azide.

[0025] The above-mentioned components were successively dissolved in 1 L of distilled water to complete the preparation.

Embodiment 3

[0027] A single-reagent serum triglyceride detection reagent with strong stability, said reagent comprising:

[0028] 50mmol / L 4-hydroxyethylpiperazineethanesulfonic acid-sodium glutamate buffer (pH=7.4), 3g / L bovine serum albumin, 0.5g / L triethanolamine dodecyl sulfate, 0.5g / L Sodium perbenzoate, 1.5g / L coenzyme FAD, 0.5g / L polyvinyl alcohol AH-26, 50mmol / L disodium edetate, 4mmol / L sodium cholate, 18mol / L magnesium sulfate heptahydrate, 2mmol / L L 4-aminoantipyrine, 4.5mmol / L N,N-dimethylaniline, 0.5g / L TritonX-100, 2KU / L lipoprotein lipase, 300KU / L glycerol kinase, 2KU / L peroxidase , 2KU / L glycerol-3-phosphate oxidase, 0.5g / L sodium azide.

[0029] The above components were successively dissolved in 1 L of distilled water to complete the preparation.

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Abstract

The invention discloses a single reagent serum triglyceride detection reagent with strong stability, and belongs to the technical field of clinical in-vitro detection. The reagent adopts a 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-sodium glutamate buffer system, so that the system has a good buffer capacity; triethanolamine lauryl sulfate, a coenzyme FAD, polyvinyl alcohol AH-26 and other substances are added in the reagent and all effectively improve the stability of the reagent. Compared with a conventional single reagent triglyceride detection reagent, the reagent has more excellent stability and is more conducive to clinical popularization and use.

Description

technical field [0001] The invention relates to a highly stable single-reagent serum triglyceride detection reagent, which belongs to the technical field of clinical in vitro detection. Background technique [0002] Triglyceride (TG) is the main component of blood lipids and an important energy supply material for the body. Triglycerides in food are digested and decomposed by pancreatic lipase in the intestinal tract, and after absorption, they are re-esterified into triglycerides and enter the blood through the lymphatic system. The liver is the main site for triglyceride synthesis, and adipose tissue is the largest triglyceride storage pool. Under pathological conditions, triglycerides synthesized and stored in various tissue cells increase significantly. Quantitative detection of triglyceride content in blood and tissue cells is a commonly used biochemical indicator in clinical, clinical basis, and basic medical biology research. [0003] At present, there are many met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/61C12Q1/28
Inventor 杨君莉罗维晓甘宜梧谭柏清李静王绮谢清华
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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