Chromatographic method for rapidly measuring content of vitamin D3

A technology of rapid determination and chromatographic method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of high cost, achieve the effects of increased detection speed, clear and obvious layering, and reduced solvent consumption

Active Publication Date: 2015-04-08
JIANGSU ALAND NOURISHMENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Chromatographic method for rapidly measuring content of vitamin D3
  • Chromatographic method for rapidly measuring content of vitamin D3
  • Chromatographic method for rapidly measuring content of vitamin D3

Examples

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Embodiment 1

[0044] Determination of vitamin D3 content in samples of Calcium Citrate 315mg+VitD3 250IU Tablet (produced by Jiangsu Aland Nutritional Products Co., Ltd.):

[0045] Reagents and preparations used in the present invention: n-hexane: chromatographically pure; isopropanol: chromatographically pure; sodium chloride: analytically pure.

[0046] (1) Standard stock solution: Accurately weigh 12.5mg of vitamin D3 reference substance in a 250ml brown volumetric flask, immediately dissolve it with n-hexane and dilute to the mark. The concentration of this solution is 50μg / mL.

[0047] Standard working solution: Precisely pipette 1.0mL of the above standard stock solution into a 100mL brown volumetric flask, immediately dissolve it with n-hexane and dilute to the mark. The concentration of this solution is 0.5μg / mL.

[0048] (2) Sample processing:

[0049] Accurately weigh 2.0g of fine powder into a 200mL brown volumetric flask, add 50mL of dimethyl sulfoxide, and tighten the stopper;...

Embodiment 2

[0065] Determination of vitamin D3 content in Calcium 600mg&VitD3 500IU Softgel (produced by Jiangsu Aland Nutritional Products Co., Ltd.):

[0066] Reagents and preparations used in the present invention: n-hexane: chromatographically pure; isopropanol: chromatographically pure; sodium chloride: analytically pure.

[0067] (1) Standard stock solution: Accurately weigh 11.25mg of vitamin D3 reference substance in a 250ml brown volumetric flask, immediately dissolve it with n-hexane and dilute to the mark. The concentration of this solution is 45μg / mL.

[0068] Standard working solution: Precisely pipette 1.0mL of the above standard stock solution into a 100mL brown volumetric flask, immediately dissolve it with n-hexane and dilute to the mark. The concentration of this solution is 0.45μg / mL.

[0069] (2) Sample processing:

[0070] Accurately weigh 2.0g of the contents into a 200mL brown volumetric flask, add 50mL dimethyl sulfoxide, and plug the stopper; put the above soluti...

Embodiment 3

[0086] Determination of vitamin D3 content in samples of calcium strip package powder (produced by Jiangsu Aland Nutrition Co., Ltd.):

[0087] Reagents and preparations used in the present invention: n-hexane: chromatographically pure; isopropanol: chromatographically pure; sodium chloride: analytically pure.

[0088] (1) Standard stock solution: Accurately weigh 13.75mg of vitamin D3 reference substance in a 250ml brown volumetric flask, immediately dissolve it with n-hexane and dilute to the mark. The concentration of this solution is 55μg / mL.

[0089] Standard working solution: Accurately pipette 1.0mL of the above standard stock solution into a 100mL brown volumetric flask, immediately dissolve it with n-hexane and dilute to the mark. The concentration of this solution is 0.55μg / mL.

[0090] (2) Sample processing:

[0091] Accurately weigh 8.0g of fine powder into a 200mL brown volumetric flask, add 50mL of dimethyl sulfoxide, and tighten the stopper; put the above solut...

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Abstract

The invention discloses a chromatographic method for rapidly measuring the content of vitamin D3, namely a method for measuring the content of vitamin D3 by using high performance liquid chromatography (HPLC); and the content of vitamin D3 in a sample containing complex components can be measured rapidly and accurately by smartly optimizing chromatographic conditions. The chromatographic method disclosed by the invention comprises the following steps: (1), preparing a reference solution; (2), processing the sample; and (3), setting liquid chromatographic conditions, wherein a chromatographic column and a silica gel column are set to 50*4.6 mm and 2.7 microns; the flow velocity is set to 2 ml/min; a mobile phase is prepared by uniformly mixing isopropanol with normal hexane according to the volume ratio of 0.5:99.5; the detection wavelength is set to 264 nm; and the sampling amount is set to 75 microliter. The chromatographic method disclosed by the invention is capable of measuring the content of vitamin D3 simply, conveniently, rapidly and accurately; the analysis time is shortened; the use amount of solvents is reduced; and the analysis cost is reduced.

Description

technical field [0001] The invention relates to a method for determining the content of vitamin D3 by using high performance liquid chromatography (HPLC). Through ingenious optimization of chromatographic conditions, the determination of the content of vitamin D3 in complex component samples is finally achieved quickly and accurately. Background technique [0002] Vitamin D3 is a vitamin D class, a steroid derivative, and it is currently believed to be a steroid hormone. It mainly helps the absorption of calcium and phosphorus, so that the level of plasma calcium and phosphorus reaches saturation; promotes growth and bone calcification, and promotes healthy teeth; maintains the normal level of citrate in the blood; prevents the loss of amino acids through the kidneys. Cholesterol in the body can be changed into 7-dehydrocholesterol, which is stored under the skin and can be transformed into vitamin D3 under sunlight and ultraviolet radiation. However, people who live in cold...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 包亚君徐小明孙青张沛霞
Owner JIANGSU ALAND NOURISHMENT
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