Novel on-line RI detection chromatography and ultrafiltration system

A detection layer and a new type of technology, applied in the field of RI online detection, can solve the problems of inconvenient disassembly of the flow cell, inaccurate flow rate, flat head phenomenon, etc., and achieve stable and controllable results, accurate flow rate, and convenient use

Inactive Publication Date: 2015-04-22
LISUI TECH SUZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Traditional differential refractive index detectors can also be connected to chromatography equipment and ultrafiltration systems for online detection of polysaccharides and other non-UV-absorbing samples, but the above-mentioned traditional differential refractive index detectors have the following disadvantages: 1) The detection range has a fixed range, which is -5120~5120μRIU. When the range of the refractive index of the mobile phase used exceeds this detection range, the full-scale flat head phenomenon will occur, and the sample cannot be detected at the full scale; 2) The detector There are two flow cells, one is the reference cell and the other is the detection cell. Before each use, the reference cell must be replaced, and the most suitable mobile phase is used as the reference, which is troublesome and time-consuming; 3 ) The flow rate can only be used at a maximum of 100ml / min. It is recommended to use a flow rate below 50ml / min. In this way, when used in pilot-scale and production-level chromatography and ultrafiltration systems, it is necessary to make a split flow into the detector. The split flow may cause the flow rate Inaccurate and inaccurate sample liquid collection; 4) The flow cell is inconvenient to disassemble, and it is difficult to clean the sample that is easy to dirty the flow cell

Method used

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  • Novel on-line RI detection chromatography and ultrafiltration system
  • Novel on-line RI detection chromatography and ultrafiltration system
  • Novel on-line RI detection chromatography and ultrafiltration system

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Such as figure 1 and Figure 5 As shown, a novel RI online detection chromatography system includes a first mobile phase liquid storage tank 11, a second mobile phase liquid storage tank 12, a first pump 21, a second pump 22, a chromatographic column 31, a new RI Online detector 40 and controller 5; the first mobile phase liquid storage tank 11 is connected to the inlet end of the first pump 21; the second mobile phase liquid storage tank 12 is connected to the inlet end of the second pump 22 The outlet port of the first pump 21 and the outlet port of the second pump 22 are connected to the inlet port of the chromatographic column 31 after being connected in parallel through the pipeline; the outlet port of the chromatographic column 31 is connected to the novel RI online detector 40 The signal output end of the novel RI online detector 40 is connected to the signal input end of the controller 5; the signal output end of the controller 5 is respectively connected to th...

Embodiment 2

[0028]Adopt a kind of novel RI online detection chromatography system of embodiment one to carry out detection experiment: in the first mobile phase liquid storage tank 11, be pure water, the second mobile phase liquid storage tank 12 is the NaCl solution of 1mol / L, to no ultraviolet The absorbed polysaccharide sample needs to be separated in the chromatographic column 31, and the absorption peak of the sample is detected with the novel RI on-line detector 40, and the difference between the elution and on-line detection process and the embodiment one is: one, in the controller Set the ratio and time of the first pump 21 and the second pump 22 in 5, wherein, the first ratio: the ratio of the first pump 21 is 100%, the ratio of the second pump 22 is 0%; the second ratio : the proportion of the first pump 21 is 90%, the proportion of the second pump 22 is 10%; the third proportion: the proportion of the first pump 21 is 80%, the proportion of the second pump 22 is 20%; the fourth ...

Embodiment 3

[0031] Such as Figure 4 and Figure 5 As shown, a novel RI online detection ultrafiltration system includes a liquid storage tank 10, a membrane bag input pump 20, a membrane bag 32, a first novel RI online detector 41, a second novel RI online detector 42 and a controller 5; the liquid storage tank 10 is connected to the inlet end of the membrane bag input pump 20; the outlet end of the membrane bag input pump 20 is connected to the inlet end of the membrane bag 32; the return output port of the membrane bag 32 Connected with the first novel RI on-line detector 41; the first novel RI on-line detector 41 is connected to the liquid storage tank 10; the filter end of the membrane bag 32 is connected to the second novel RI on-line detector 42; The signal output end of the first novel RI online detector 41 and the signal output end of the second novel RI online detector 42 are respectively connected with the signal input end signal of the controller 5; the signal output end of t...

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Abstract

The invention discloses a novel on-line RI detection chromatography and ultrafiltration system, relates to the field of on-line RI detection and aims to solve the problem of disadvantages caused by that a traditional differential refraction detector can also be connected to chromatographic equipment and an ultrafiltration system in use. A novel on-line RI detection chromatography system comprises a first mobile-phase liquid storage tank, a second mobile-phase liquid storage tank, a first pump, a second pump, a chromatographic column, a novel on-line RI detector and a controller. A novel on-line RI detection ultrafiltration system comprises a liquid storage tank, a membrane bag input pump, a membrane bag, a first novel on-line RI detector, a second novel on-line RI detector and a controller. The novel on-line RI detection chromatography and ultrafiltration system disclosed by the invention is applicable to procedures of on-line detection of chemical industry, biochemical separation analysis and production and the like, also has the advantages of being simple in structure, convenient to use, stable and controllable in result and the like, causes no full-scale flat end, needs no flow splitting, and is accurate in flow speed and sample liquid collection.

Description

technical field [0001] The invention relates to the field of RI on-line detection, in particular to a novel RI on-line detection chromatography and ultrafiltration system. Background technique [0002] Traditional differential refractive index detectors can also be connected to chromatography equipment and ultrafiltration systems for online detection of polysaccharides and other non-UV-absorbing samples, but the above-mentioned traditional differential refractive index detectors have the following disadvantages: 1) The detection range has a fixed range, which is -5120~5120μRIU. When the range of the refractive index of the mobile phase used exceeds this detection range, the full-scale flat head phenomenon will occur, and the sample cannot be detected at the full scale; 2) The detector There are two flow cells, one is the reference cell and the other is the detection cell. Before each use, the reference cell must be replaced, and the most suitable mobile phase is used as the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 周胜林丹
Owner LISUI TECH SUZHOU
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