Method of treatment

A stem cell and disease technology, applied in pharmaceutical formulations, medical raw materials derived from mammals, cells of the nervous system, etc., can solve problems such as no possibility of designing effective treatments

Inactive Publication Date: 2015-04-22
MEDVET SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no direct knowledge of the "causes and effects" of the disease condition, making it virtually impossible to design effective treatments

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0106] Materials and methods

[0107] Mice. 14-3-3ζ carrying a gene trap construct containing a Geo reporter gene Gt(OST062)Lex and 14-3-3ζ Gt(OST390)Lex Mutant mice were obtained from Lexicon Genetics ES cell lines OST062 and OST390, respectively. 14-3-3ζ Gt(OST062)Lex The gene trap vector in mouse inserts the first intron of 14-3-3ζ, while 14-3-3ζ Gt(OST390)Lex Gene trap vector insertion in the second intron of 14-3-3ζ in mouse. ES cell lines were expanded and injected into SV129 blastocysts. The resulting germline transmission males were maintained on the SV129 background or backcrossed within 6 generations into C57 / Bl6 and BA1, BC backgrounds. qRT-PCR and western blots from whole tissue samples were employed to confirm complete KO of this gene in these mouse strains. The 14-3-3C genotype was determined by PCR amplification of genomic tail DNA using the primers detailed in Supplementary Table 1. The wild-type allele was amplified by 288bp (14-3-3ζ Gt(OST062)Lex ) or ...

Embodiment 2

[0139] Demonstration that NRP2-positive neuronal precursors generate hippocampal regions

[0140] To identify mature neurons derived from neuronal precursors and expressing Nrp1 or Nrp2, Nrp1 and Nrp2 line-traced mice were generated. To this end, Cre / RFP or Cre / GFP have been placed under Nrp1 or Nrp2 promoter expression ( Figure 16 ). Studies using these mice (from n=2 experiments, Figure 17 ) show for the first time that hippocampal neurons are derived from Nrp2-expressing neural stem cells.

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Abstract

The present invention relates generally to a method of regenerating the hippocampus in a mammal and agents for use therein. More particularly, the present invention provides a method of regenerating the hippocampus in a mammal by administering a sub-population of neural crest stem cells. The method of the present invention is useful in the treatment of conditions characterised by a defective hippocampus, such as neuropsychiatric disorders.

Description

field of invention [0001] The present invention generally relates to methods of regenerating the hippocampus in mammals and reagents for use in such methods. More specifically, the present invention provides methods for regenerating the hippocampus in mammals by administering a subset of neural crest stem cells. The methods of the invention are useful in the treatment of disorders characterized by defects in the hippocampus, such as neuropsychiatric disorders. Background of the invention [0002] A detailed bibliography of publications by the authors in this specification is grouped alphabetically at the end of the specification. [0003] Reference in this specification to any prior art is not to be taken as, and should not be taken as, an acknowledgment or any form of suggestion that such prior art forms part of the common general knowledge in Australia. [0004] Schizophrenia is one of the most crippling and emotionally destructive disorders known to humans. Unfortunate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0793C12N5/0797A61K45/00A61P25/18A61P25/28
CPCA61K35/36A61K35/12A61K35/32A61K35/30C12N5/0623A61P25/00A61P25/18A61P25/24A61P25/28
Inventor A·洛佩兹Q·施瓦茨
Owner MEDVET SCI
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