A method for extracting capsular polysaccharides targeting intestinal bacteria
A technology of capsular polysaccharides and intestinal bacteria, applied in the field of biopharmaceuticals, can solve problems such as environmental pollution and human injury
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Embodiment 1
[0029] Salmonella in Salmonella typhi fermentation broth with batch numbers 20140201 and 20140202 were purchased from the China Medical Bacteria Collection Management Center of the China National Institutes for Food and Drug Control. The Salmonella typhi fermented broths with batch numbers 20140201 and 20140202 were prepared according to the same process parameters as "Chinese Pharmacopoeia" (2010 edition).
[0030] The steps and results of extracting the typhoid Vi polysaccharide of the Salmonella typhi fermented liquid whose batch numbers are 20140201 and 20140202 are as follows:
[0031] (1) Add a concentration of 37% (ml / ml) formaldehyde solution to the Salmonella typhi fermentation broth to sterilize, centrifuge at 8000r / min and 8°C for 20min to remove the bacterial sediment, and collect the supernatant of the culture broth; the amount of formaldehyde added Be: every liter of Salmonella typhi fermented liquid adds 5 milliliters of concentrations and is the formaldehyde so...
Embodiment 2
[0047] Salmonella in Salmonella typhi fermentation broth with batch numbers 20140303 and 20140304 were purchased from the China Medical Bacteria Collection Management Center of the China National Institutes for Food and Drug Control. The Salmonella typhi fermented broths with batch numbers 20140303 and 20140304 were prepared according to the same process parameters as "Chinese Pharmacopoeia" (2010 edition).
[0048] The steps and results of extracting the typhoid Vi polysaccharide of the Salmonella typhi fermented liquid whose batch numbers are 20140303 and 20140304 are as follows:
[0049] (1) Add a concentration of 37% (ml / ml) formaldehyde solution to the Salmonella typhi fermentation broth to sterilize, centrifuge at 8000r / min and 8°C for 20min to remove the bacterial sediment, and collect the culture supernatant; the amount of formaldehyde added Be: adding 20 milliliters of concentration to be the formaldehyde solution of 37% (ml / ml) for every liter of Salmonella typhi fer...
Embodiment 3
[0062] Embodiment 3 (comparative test)
[0063] In order to better illustrate the present invention, four batches of 20140305, 20140306, 20140407, and 20140408 were extracted from the fermentation of Salmonella typhi respectively, and the fermentation liquid of each batch was divided into two parts -1 and -2 on average, so as to carry out better Compared. 20140305, 20140306, 20140407, and 20140408 four batches of Salmonella typhi in fermentation were purchased from the Chinese Medical Bacteria Preservation Management Center of the Chinese Academy of Food and Drug Control. For the four batches of Salmonella typhi fermentation broth with batch numbers 20140305, 20140306, 20140407, and 20140408, they were all prepared according to the same process parameters of "Chinese Pharmacopoeia" (2010 edition).
[0064] - One batch was extracted by the method of the present invention according to the following steps, and 20140305-1, 20140306-1, 20140407-1, 20140408-1 were prepared respecti...
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