Mushroom liquefied strain cultivation method

Abstract

The invention discloses a mushroom liquefied strain cultivation method. The method sequentially comprises the steps of 1, strain preparation, wherein high-temperature and high-pressure sterilization is conducted on a special mushroom liquefied strain culture medium, the culture medium is inoculated into mushroom mother strain under the sterile condition after sterilization, and cultivation is conducted for 19-26 days in dark at a temperature between 17 DEG C and 25 DEG C; 2, liquefied strain preparation, wherein obtained special liquefied solid strain is diluted to obtain mushroom liquefied strain. The special liquefied solid strain high in purity and stability can be obtained with the method, so that efficient mycelium multiplication is achieved, and the method can be applied to liquidation of wood-destroying fungi including shiitake mushroom, agaric, flammulina velutipes, pleurotus eryngii, pleurotus geesteranus, pholiota nameko and hypsizigus marmoreus.

Description

technical field [0001] The invention relates to a special strain formula and cultivation method for mushroom liquefaction. Background technique [0002] Edible fungus is one of the advantageous industries with the most modern agricultural development characteristics in China. This is not only because China is the world's largest producer and consumer of edible fungi, with a wide range of edible fungus production, but more importantly, the edible fungus industry is a good carrier and prominent position in the industrialization of biotechnology and the sustainable development of agriculture. At present, my country's edible fungus industry system has many factors that are not suitable for the global market economy, the most important of which are the intensification of industrial operations, low degree of specialization, backward production factors, imperfect production technology, and lack of key technology support. The most representative one is the intensive and efficient br...

AI Technical Summary

Problems solved by technology

Remarks: The liquid fermentation strain process theoretically has a high purity of the strain, and the bacterial contamination rate of large-scale fermentation production is between 1% and 5% (in a 3-stage fermenter), and the bacterial contamination (mainly bacterial) is often In the later stage of fermentation, it is difficult to find out by conventional on-line inspection, which leads to production accidents caused by the inoculation of liquid fermentation strains and the contamination of large quantities of mushroom bags, which is a painful lesson.

In addition, because the cultivation of liquid strains requires nutrients such as high-concentration organic nitrogen and sugar sources, these ingredients remain in the bacterial liquid and are inserted into the fruiting bag. Since bacteria are inevitably brought in during operation, the residual nutrients It becomes a breeding ground for miscellaneous bacteria, which increases the risk of contamination after inoculation of the fruiting bag

This is the main problem that liquid fermentation strains cannot be applied to the production of edible fungi on a large scale

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