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Sorbent and hemoperfusion device for treating Gram infection

A technology of gram bacteria and hemoperfusion device, which is applied in the field of hemoperfusion device, can solve problems such as limited application, and achieve the effects of reducing shedding, good treatment of gram bacteria infection, and improving immobilization strength

Active Publication Date: 2017-02-01
佛山市博新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Melittin has a strong inhibitory effect on Gram-positive and negative bacteria, but it has a strong hemolytic activity, which limits its clinical application

Method used

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  • Sorbent and hemoperfusion device for treating Gram infection
  • Sorbent and hemoperfusion device for treating Gram infection
  • Sorbent and hemoperfusion device for treating Gram infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Cation exchange resin immobilized Nisin element

[0032] Take 10g of cation exchange resin (pore diameter 100nm, specific surface area 60㎡ / g, ion exchange capacity is 10mmol / g), add 2.5g Nisin element aqueous solution 50mL, and shake at room temperature for 8 hours. Then wash it with injection water to get S1.

Embodiment 2

[0033] Example 2 Long-chain modified cation exchange resin immobilized Nisin element

[0034] 2-1 Long chain modification of cation exchange resin

[0035] Take 10g of cation exchange resin (pore diameter 100nm, specific surface area 60㎡ / g, ion exchange capacity is 10mmol / g), add 30mL 10% hexamethylenediamine solution, adjust pH to 4.8, add 0.4g NHS(N-hydroxysuccinyl Imine), then slowly add 0.5 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), and react with shaking at 25°C for 4 hours. After the reaction is completed, it is washed with alcohol and purified water.

[0036] Then add 30mL 10% adipic acid solution, adjust the pH to 4.8, add 0.6g NHS (N-hydroxysuccinimide), then slowly add 0.5g 1-(3-dimethylaminopropyl)-3 -Ethylcarbodiimide hydrochloride (EDC), shake and react at 25°C for 4 hours. After the reaction is completed, it is washed with alcohol and purified water.

[0037] 2-2 immobilized Nisin element

[0038] Take 10 g of the modified cation exchange...

Embodiment 3

[0039] Example 3 Long-chain modified cation exchange resin immobilized polylysine

[0040] 3-1 Long-chain modification of cation exchange resin

[0041] Same as Example 2 Step 2-1

[0042] 3-2 immobilized polylysine

[0043] Take 10 g of the modified cation exchange resin, add 50 mL of an aqueous solution of 2.5 g of polylysine, and then add 0.2 g of EDC, and shake at room temperature for 8 hours. Then rinse with injection water. The gram bacteria adsorbent S3 is obtained.

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Abstract

The invention discloses an adsorbent for treating gram bacterial infection and a blood perfusion device. A carrier of the adsorbent is cation exchange resin or activated polystyrene divinyl phenyl resin, a ligand is Nisin, polylysine or melittin analogue. By selecting a carrier type and immobilizing the Nisin, the polylysine or the melittin analogue with specific effect on the gram bacteria, a purpose of treating the gram bacterial infection is achieved. The blood perfusion device comprises a perfusion machine which is filled with the adsorbent for treating gram bacterial infection, and further comprises a perfusion machine which is filled with an adsorbent for adsorbing inflammatory factors, so that the inflammatory factors in the blood can be effectively removed to achieve effects of relieving and treating. Furthermore, the blood perfusion device comprises a device for dropwise adding sodium lactate injection, so that cooperative effect is generated on inhibition and adsorption of the gram bacteria, and a relatively good effect of treating the gram bacterial infection is achieved.

Description

Technical field [0001] The invention relates to the field of blood purification and medical materials, in particular to an adsorbent used for treating gram infections and a blood perfusion device containing the adsorbent. Background technique [0002] Gramella [0003] Gram bacteria include Gram-negative bacteria and Gram-positive bacteria. Most of the intestinal bacteria are Gram-negative bacteria. They produce endotoxins and make people sick by endotoxins. Most pyogenic cocci are Belonging to Gram-positive bacteria, they can produce exotoxin to make people sick. Common Gram-positive bacteria are: Staphylococcus, Streptococcus, Pneumococcus, Bacillus anthracis, Diphtheria, Tetanus Bacillus, etc. In the past, many researchers paid more attention to the study of gram-negative bacteria infection and endotoxemia, but they had little interest in gram-positive bacteria sepsis. [0004] Large-scale epidemiological data show that sepsis and septic shock caused by gram-positive bacteria h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26A61M1/38
CPCA61M1/3612A61M1/3633A61M1/38B01J20/26B01J2220/44B01J2220/4812
Inventor 李设桥姜建明
Owner 佛山市博新生物科技有限公司
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