A method and system for dynamically preparing biochips with linear array nozzles

Abstract

The invention relates to a method for dynamically preparing a biochip by using linear-array sprayers, which implements dynamic sample dotting of the biochip, shortens the sample dot preparation time and ensures the preparation consistency of the sample dots. The linear-array sprayers are longitudinally moved to sequentially implement dynamic spray printing on the same sample dot by the multiple sprayers, thereby completing the sample dot preparation at high speed. The method changes the traditional sample dotting mode which needs static and repeated spraying due to small volume of the sample dotting sprayers. The linear-array sprayers are longitudinally moved, so that the plurality of identical sprayers sequentially perform spray printing and sample dotting on each sample dot, thereby avoiding the volume difference of the sprayed liquid drops caused by different sprayer hole diameters, and ensuring the uniformity of the volume of the prepared biological sample dot. The linear-array sprayers are longitudinally moved to sequentially perform dynamic sample dotting, thereby effectively lowering the quantity of the sprayers, reducing the working space of the sample dotting apparatus and lowering the manufacturing cost of the sample dotting apparatus.

Description

Technical field: [0001] The invention relates to a method and a system for dynamically preparing a biochip with a line array nozzle. Background technique: [0002] Biochips are mainly characterized by the existence of microarrays of sample points. The density of microarrays ranges from tens to tens of thousands of points per square centimeter. There are multiple square arrays on one chip, and the sample points in the square arrays are different. Some samples Click to copy several times. The preparation method of the biochip mainly uses the biochip spotting instrument (or also called "sample spotting device") to place the DNA, RNA, protein and other biological components to be studied or tested in a desired matrix in a high-density and It is fixed on solid-phase dense carriers such as silicon chips, glass chips or pottery chips in a high-precision way, and then forms a biomolecular lattice. [0003] The non-contact spotting method is the main development direction of spotti...

AI Technical Summary

Problems solved by technology

This method not only has a cumbersome spotting process, but also because the volume of the sample droplet sprayed out by a single nozzle is too small to meet the requirements of the sample point, so the static and multiple spraying methods are often used to meet the requirements, but the sample pointing speed of this method Low; in order to increase the sampling speed, some enterprises use multi-nozzle (needle) combination to increase the sampling speed, but due to the processing accuracy, the aperture diameter of each nozzle is different. Using this sampling method is easy to cause error accumulation, resulting in the prepared The sample point uniformity of the biochip is low; moreover, the combination of multiple nozzles (needles) results in a large working space for the spotting equipment and high production costs, so that it is difficult for most scientific research institutions to accept

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