A liquid-based preservation solution for exfoliated cells, its method and kit for making sheets

Abstract

The invention discloses an exfoliative cell sap base preserving fluid, a method thereof for flaking and a kit. The exfoliative cell sap preserving fluid comprises the following components by massic volume percentage: 3.8-4.2% of paraformaldehyde, 0.8-1.5% of N-methyl-N-cyclohexyl-2-amino-3,5-bromhexine, 0.8-1.5% of glacial acetic acid, 0.1-0.2% of acetylcysteine, 0.75-0.85% of sodium chloride, 0.01-0.03% of potassium chloride, 0.12-0.15% of disodium hydrogen phosphate, 0.025-0.03% of monopotassium phosphate, and the balance of water; and pH is regulated to 7.2-7.8. The exfoliative cell sap base preserving fluid is small in shrinking to the exfoliative cell, and suitable for storage for a long time; the cell coated by the mucus in the sample is successfully separated, the positive cell stacking and loss are avoided, and the effective cell number is improved; and the preserving fluid can be used for observing whether the occult blood symptom is existent.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an exfoliated cell liquid-based preservation solution, its method for making tablets and a kit. Background technique [0002] Colorectal cancer is a common malignant tumor that endangers human life, and its incidence rate ranks third among malignant tumors in the world. In my country, its morbidity and mortality are increasing year by year. It is often late when patients have symptoms and see a doctor, and the curative effect is poor. If it can be detected and treated at an early stage, the effect will be better. Currently, the clinical detection techniques for colorectal cancer mainly include: (1) fecal occult blood detection; (2) endoscopic examination; (3) fecal exfoliation cytology detection. Among them, fecal exfoliation cytology detection has been widely used in clinical practice due to its simplicity, high safety, low equipment requirements, low cost, and less pain to patient...

AI Technical Summary

Problems solved by technology

[0004] The invention overcomes the defects that the existing exfoliated cell detection liquid-based preservation solution cannot be combined with fecal occult blood detection, multiple steps are cumbersome to process fecal exfoliated cells, and damages hemoglobin, and provides a liquid-based exfoliated cell preservation solution. Cell liquid-based preservation solution can fix exfoliated cells, remove mucus protein, and retain red blood cells for observation of occult blood symptoms

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