Application of annosquatin A in preparation of pesticide preparation

A technology for pesticide preparation and methanol is applied in the field of preparation of succinyl lactone compounds, which can solve the problems of complex method, low yield, and no known preparation method for annoskotin first, and achieves low environmental pollution and good quality. The effect of poisoning activity

Inactive Publication Date: 2015-05-27
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
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  • Application Information

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Benefits of technology

This patented technology helps researchers identify which chemicals are most effective at killing or repelling harmful insects such as ants moths (Another name: Phytophthora litura) and tsetse-ring bacteria called Rhizoctonia solani. It also suggests making certain types of substances like this one more selective against specific species of plants without causing damage on other crops when applied alone.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding ways to make chemically synthetic substances that can kill or control animal and plant disease without causing damage to their ecosystems.

Method used

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  • Application of annosquatin A in preparation of pesticide preparation
  • Application of annosquatin A in preparation of pesticide preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 2kg of custard apple seeds, crush them, and percolate the coarse powder twice with 5 times the amount of ethyl acetate, combine the percolation liquid, recover under reduced pressure below 60°C to obtain the concentrated liquid, let it stand overnight, and centrifuge at 3000r / min to obtain the obtained product The upper layer (123g), mixed with 120g of silica gel, was subjected to 500g silica gel column chromatography, and was eluted with petroleum ether-ethyl acetate gradient, the ratios were 10:1, 5:1, 2:1, 1:1, 0:1 , 10 fractions were collected for each ratio, each fraction was 500 mL, tracked by thin-layer chromatography, 33-40 fractions were combined, concentrated and dried under reduced pressure at less than 60°C. Recrystallize with 5 times the amount of petroleum ether-ethyl acetate (5:1) to obtain 0.2 g of refined anoxactine A. Its molecular weight was determined to be 622.4 by 1H-NMR data and MS, and it was determined to be annulotide anorostecidine A.

Embodiment 2

[0025] Take 1.8kg of custard apple seeds, crush them, and pass the coarse powder through 20 meshes, and perform supercritical carbon dioxide extraction under the conditions of extraction temperature 35°C, extraction pressure 30Mpa, extraction time 3 hours and entrainer ethanol consumption 180mL. The extract was obtained, which was recovered under reduced pressure below 60°C to obtain 98 g of concentrated liquid. After mixing 100g of silica gel, carry out 500g of silica gel column chromatography, and use chloroform-methanol gradient elution, the ratio is 100:1, 50:1, 25:1, 10:1, each ratio collects 10 fractions, each fraction is 500mL , traced by thin layer chromatography, combined 15-21 fractions, concentrated and dried under reduced pressure at less than 60°C. Recrystallize with 5 times the amount of chloroform-methanol (50:1) to obtain 0.23 g of refined anoxetine A. Its molecular weight was determined to be 622.4 by 1H-NMR data and MS, and it was determined to be annulotide...

Embodiment 3

[0026] The biological activity of embodiment 3 Anoxetine A

[0027] 1. Determination of the contact effect on bean aphid:

[0028] Use a micro-dropper to drip 10% distilled acetone aqueous solution containing different concentrations of anoxactin A on the abdomen and back of the bean aphid, and the amount of dripping per insect is 0.05 μL. After the dripping was completed, each was put into a biochemical incubator for cultivation. After 24 hours, the death situation was checked and the death rate was calculated. Immersion with 10% acetone distilled water was used as a control. The mortality rate of the control group was less than 10% for effective determination, and the mortality rate of the treatment group was corrected by Abbott's formula. Measured 3 times in parallel. The test results were analyzed using SPSS 13.0 to calculate the lethal concentration LC 50 .

[0029] 2. Determination of contact killing effect on Tetranychus cinnabarinus:

[0030] Paste the double-si...

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Abstract

The invention discloses an annona squamosa lactone compound annosquatin A prepared from an annona squamosa seed and capable of preventing and controlling agricultural pests. A large number of biological activity assay results show that the annona squamosa lactone compound annosquatin A has poisonous activities in different degrees to the aphis craccivora and cinnabar tetranychid, especially has better poisonous activity to the aphis craccivora than abamectin, and the annosquatin A is extracted from the annona squamosa, the pollution to the environment is small, the annosquatin A is green and environment-friendly, and can be used for developing a new pesticide preparation for preventing and controlling agricultural pests.

Description

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Claims

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Application Information

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Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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