Method for extracting rice germ protein
An extraction method and rice germ technology, applied in the field of extraction, can solve the problems of focusing on protein content, single protein nutrient composition, unfavorable human health, etc., and achieve the effects of convenient operation, high nutrient composition, and high-quality and high-efficiency products.
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[0014] A method for extracting rice germ protein, comprising the steps of:
[0015] (1) Weigh the degreased sample, add water to dissolve it, preheat it in a water bath, put it into a constant temperature magnetic stirrer and vibrate to dissolve the protein in the sample solution, centrifuge at 4000r / min for 20 minutes, and collect the supernatant, namely for albumin;
[0016] (2) Take the above-mentioned residue extracted by water, add 6% sodium chloride solution, after fully dissolving, collect the supernatant by the same method, which is globulin;
[0017] (3) Add the residue to 70% ethanol solution, dissolve, collect the supernatant, which is the alcohol-soluble protein:
[0018] (4) Add 0.4% sodium hydroxide to the remaining precipitate, fully dissolve, and prepare gluten in the same way.
[0019] The amount of water added in the present invention is 75mL, the extraction temperature is 40°C, and the extraction rate is 51.01% for 30min; the sodium chloride solution is 62...
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