Supercharge Your Innovation With Domain-Expert AI Agents!

A kind of rapid tissue culture method of golden camellia

A technology of golden camellia and golden camellia tea, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of not seeing the species with golden flower color, etc., and achieve the benefits of industrial production and automatic monitoring, shortening the cycle of cultivation time, the effect of reducing power consumption

Inactive Publication Date: 2017-04-05
产学研共同体(山东)科技成果转化有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previously, people had not seen species with golden flowers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for rapid tissue culture of camellia japonica, comprising the steps of:

[0027](1) Disinfection of explants: at 1 o'clock in the evening in June, pick golden camellia tea leaves that are light green in color, complete, and free from diseases and insect pests, then use 20% dimethoate emulsion to dilute 400 times and soak for 5 minutes, take them out and put them into an ultra-clean workbench Use 4% vitamin C aqueous solution to soak for 30 minutes, then sterilize with 3% hypochlorous acid for 5 minutes, then rinse with sterile water for 3 times, and finally use sterile filter paper to dry the sterile water on the surface of the golden camellia tea;

[0028] (2) Callus culture: Use a scalpel to cross-cut leaf strips with a length of 15 mm and a width of 10 mm along the stem position of the golden camellia tea, and put 3 leaf strips into the callus culture medium In a petri dish with a specification of 90×15mm, lightly press the leaf strips, culture in the dark a...

Embodiment 2

[0032] A method for rapid tissue culture of camellia japonica, comprising the steps of:

[0033] (1) Disinfection of explants: at 5:00 p.m. in July, pick golden camellia tea leaves that are light green in color, complete, and free from diseases and insect pests, then use 40% dimethoate emulsion to dilute 600 times and soak for 10 minutes, take them out and put them into an ultra-clean workbench Use 4% vitamin C aqueous solution to soak for 30 minutes, then sterilize with 3% hypochlorous acid for 10 minutes, then rinse with sterile water for 5 times, and finally use sterile filter paper to dry the sterile water on the surface of the golden camellia tea;

[0034] (2) Callus culture: Use a scalpel to cross-cut the leaf strips with a length of 18 mm and a width of 15 mm along the stem position of the golden camellia tea, and put 5 leaf strips into the callus culture medium In a petri dish with a specification of 90×15mm, lightly press the leaf strips, culture in the dark at 31°C f...

Embodiment 3

[0038] A method for rapid tissue culture of camellia japonica, comprising the steps of:

[0039] (1) Disinfection of explants: at 1:40 p.m. in June, pluck golden camellia tea leaves with light green color, completeness, and no damage by diseases and insect pests, then use 25% dimethoate emulsion to dilute 450 times of liquid and soak for 6 minutes, put into super The clean workbench is soaked in 4% vitamin C aqueous solution for 30 minutes, then disinfected with 3% hypochlorous acid for 6 minutes, then rinsed with sterile water for 4 times, and finally use sterile filter paper to dry the sterile water on the surface of the golden camellia tea;

[0040] (2) Callus culture: Use a scalpel to cross-cut leaf strips with a length of 16 mm and a width of 11 mm along the stem position of the golden camellia tea, and put 4 leaf strips into the callus culture medium In a petri dish with a specification of 90×15mm, lightly press the leaf strips, culture in the dark at 30°C for 9 days, an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a rapid tissue culture method for golden camellia. The rapid tissue culture method is characterized by comprising the following steps: (1) disinfection of explants, (2) callus culture, (3) callus bud differentiation and (4) bud rooting. According to the rapid tissue culture method for golden camellia, the propagation cost can be reduced, golden camellia is short in growth cycle, high in propagation rate and convenient to manage, and the method is beneficial to industrialized production and automatic monitoring; and manpower, material resources and lands for field crops are greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of tissue culture production, and relates to a rapid tissue culture method of camellia japonica. Background technique [0002] Camellia Camellia belongs to the family Theaceae and Camellia genus, and it is twin sisters with Tea, Camellia, Camellia oleifera, Camellia oleifera, and Camellia sasana. The flowers of Camellia japonica are golden yellow, dazzling, as if coated with a layer of wax, crystal clear and oily, as if translucent. Camellia chrysalis grows solitary in the leaf axils. When the flowers bloom, there are cup-shaped, pot-shaped or bowl-shaped flowers, which are delicate and colorful, beautiful and elegant. Previously, people had not seen species with golden flowers. In 1960, Chinese scientists first discovered a golden yellow camellia in Nanning, Guangxi, which was named Camellia Camellia. The oriental magic tea, which is called miraculous abroad, is known as "the giant panda in the plant kin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张莘蔓韦玲菊陈程罗生芳
Owner 产学研共同体(山东)科技成果转化有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com