A method for measuring the starch content of chestnut
A chestnut starch and content technology, which is applied in the measurement of color/spectral characteristics, material analysis by observing the impact on chemical indicators, and analysis by making materials undergo chemical reactions, can solve the problem of affecting the accuracy and precision of measurement results , Unable to accurately measure starch content, unable to rule out sugar interference and other problems, to achieve high work efficiency, improved accuracy, and strong specificity
Inactive Publication Date: 2017-07-25
SHANDONG INST OF POMOLOGY
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Problems solved by technology
In the determination of chestnut quality, starch is often an indispensable measurement index, but because starch and sugar exist at the same time, and both belong to polysaccharides, it is easy to be interfered by other sugars when starch is measured alone, thus Affect the accuracy and precision of the measurement results
As far as the existing starch determination methods, such as anthrone method, enzyme hydrolysis method, acid hydrolysis method, etc., or because of the inability to rule out sugar interference, or because of complicated operations, it is impossible to accurately determine the concentration of starch and total sugar in fruits such as chestnuts that contain both starch and total sugar. starch content
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Embodiment 1
[0032] 1. Prepare the main instruments and reagents
[0033] (1) Instruments: pulverizer; oven; 0.125mm sample sieve; water bath; visible spectrophotometer; 150ml triangular flask; 100ml volumetric flask; 75mm glass funnel;
[0034] (2) Reagents: 0.5% α-amylase; ether; 85% ethanol; distilled water; 2.0g L-1 anthrone sulfate (0.5g anthrone dissolved in 250ml concentrated sulfuric acid); I-KI solution (3.6g KI, 1.3g of I dissolved in 20ml of distilled water).
[0035] 2. Standard curve creation
[0036] (1) Prepare 250mg·L-1 starch standard solution: Accurately weigh 250mg of starch and place it in a 150ml Erlenmeyer flask, then add 50ml of distilled water. Put the triangular flask in a water bath and boil it for 15 minutes, then take it out and cool it down. Add 10ml of 0.5% α-amylase solution into the cooled Erlenmeyer flask, and incubate in a 60°C water bath for 1 hour. Then use the I-KI solution to detect the solution in the Erlenmeyer flask. If the blue color does not a...
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The invention relates to a method for measuring the starch content of Chinese chestnut and belongs to the technical field of food detection. The method comprises the following steps: performing drip washing on a sample to be measured by use of diethyl ether and ethanol, next, performing enzymatic hydrolysis on the sample to be measured by use of an alpha-amylase, and then reacting anthrone sulfate with the enzymatic hydrolysis product, and then measuring the light absorption value of the product under the wavelength of 620nm. The light absorption value of a standard starch solution is detected by use of the method and a standard curve is established. The light absorption value of the same is compared with the standard curve, and then the starch concentration is calculated by use of a formula. The method is high in specificity and suitable for measuring fruits containing lots of starch and other saccharides simultaneously; secondly, the interference of other saccharide substances in the sample can be removed thoroughly and the accuracy of the experimental result can be improved; thirdly, the operation process is simple, the working efficiency is high and the precision of the experimental result can be improved.
Description
technical field [0001] The invention relates to a method for measuring the starch content of chestnuts, belonging to the technical field of food detection. Background technique [0002] Starch and total sugar are important components of the quality of chestnut nuts, both exist in chestnut pulp, and with the prolongation of storage time, starch is gradually converted into sugar substances. In the determination of chestnut quality, starch is often an indispensable measurement index, but because starch and sugar exist at the same time, and both belong to polysaccharides, it is easy to be interfered by other sugars when starch is measured alone, thus Affect the accuracy and precision of the measurement results. As far as the existing starch determination methods, such as anthrone method, enzyme hydrolysis method, acid hydrolysis method, etc., or because of the inability to rule out sugar interference, or because of complicated operations, it is impossible to accurately determin...
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IPC IPC(8): G01N21/78G01N21/31
Inventor 田寿乐沈广宁孙晓莉
Owner SHANDONG INST OF POMOLOGY