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Capillary isokinetic microchannel electrophoresis chip

An electrophoretic chip and microchannel technology, which is applied in the direction of material analysis, measuring devices, and instruments through electromagnetic means, can solve problems such as poor measurement accuracy, long test length, and large resistance loss, and achieve the effect of ensuring accuracy

Inactive Publication Date: 2017-02-22
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to overcome the technical defects of the existing capillary electrophoresis equipment such as long test length and large resistance loss resulting in poor measurement accuracy, the present invention discloses a capillary isokinetic microchannel electrophoresis chip

Method used

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  • Capillary isokinetic microchannel electrophoresis chip

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Embodiment Construction

[0021] The specific embodiment of the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0022] The capillary isokinetic microchannel electrophoresis chip of the present invention includes a chip body 1, and a sampling capillary 2 and a detection capillary located on the chip body. The middle part of the sampling capillary and one end of the detection capillary are connected vertically in a T shape.

[0023] Such as figure 1 as shown, figure 1 The vertical direction is the sampling capillary, the horizontal direction is the detection capillary, and the two are connected vertically in a T shape. figure 1 In order to facilitate sample injection and sampling, a first solution pool is provided at both ends of the sampling capillary, and a second solution pool is provided at the end of the detection capillary not connected to the sampling capillary. The size of the first solution pool and the second solution pool can be ca...

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Abstract

The capillary isokinetic microchannel electrophoresis chip of the present invention includes a chip body, including a sampling capillary and a detection capillary located on the chip body, the middle part of the sampling capillary and one end of the detection capillary are vertically connected in a T shape. Using the capillary isokinetic microchannel electrophoresis chip of the present invention, the etched chip is used to replace the ordinary capillary, so that the electrophoresis path is significantly shortened and more regular; the T-shaped capillary is used to make it more convenient to inject the leading, trailing buffer and sample separately, and It can effectively shorten the electrophoresis path and reduce the resistance loss of the fluid along the way, and ensure the accuracy of pollutant monitoring to a greater extent.

Description

technical field [0001] The invention belongs to the field of chemical testing equipment, and relates to a solution component measuring device, in particular to a capillary isokinetic microchannel electrophoresis chip. Background technique [0002] Capillary isotachophoresis (CITP) is a discontinuous electrophoresis mode based on the difference in ion mobility. After the detection sample is fluorescently labeled, it is injected between the leading electrolyte (LE) and the trailing electrolyte (TE). Its mobility allows various charged particles to be separated, and after reaching a steady state, it advances at the same speed as the leading electrolyte. This method has high electrophoresis efficiency, short separation time, good reproducibility, and has a compression effect on the zone, so the sensitivity and specificity are higher than those of ordinary zone electrophoresis. It has the characteristics of fast detection speed, less sample and reagent consumption, and low analy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447
Inventor 郑庭辉丁冠乔郭勇童启邦
Owner SICHUAN UNIV