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Probiotic Microcapsules for Feed and Its Application

A technology of microcapsules and probiotics, applied in the fields of application, animal feed, animal feed, etc., can solve the problems of poor gastric environmental tolerance, unfavorable industrialization and application of microcapsule products, and destruction of lactic acid bacteria, and achieve the purpose of inhibiting the growth of pathogenic bacteria, Improve the feeding effect of animals and enhance the effect of gastric acid resistance

Active Publication Date: 2018-02-02
SHANDONG JIANYUAN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] To sum up, the existing microcapsule coating technology will cause greater damage to lactic acid bacteria during its implementation, or the microcapsules produced have poor tolerance to the gastric environment, which is not conducive to Industrialization and application of microcapsule products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The specific breeding process of Lactobacillus plantarum tlj-2014 of the present invention is as follows:

[0033] 1. DES mutagenesis selection

[0034] 1) Take the L-ring of Lactobacillus plantarum on the inclined surface of the test tube on the ultra-clean bench, insert it into a 250mL Erlenmeyer flask filled with 50mL medium MRS (no agar, glucose 20g / L), and culture at 200rpm at 37°C for about 12h. Make the bacteria in the early logarithmic growth stage.

[0035] 2) Take 5 mL of the bacterial liquid, centrifuge at 5000 rpm for 10 minutes to collect the bacterial cells, and wash twice with normal saline.

[0036] 3) Dilute with pH7.0 phosphate buffer to 107 / mL bacterial suspension.

[0037] 4) Take 32mL of pH7.0 potassium phosphate buffer, 8mL of bacterial suspension, and 0.4mL of DES in a 150mL Erlenmeyer flask pre-placed in the rotor and mix thoroughly so that the final concentration of DES is 1% (v / v).

[0038] 5) React in a shaker at 37° C. at 150 rpm for 30 mi...

Embodiment 2

[0061] A probiotic microcapsule product is composed of wall material, Lactobacillus plantarum, stachyose, and hickory chick enzymolysis powder.

[0062] The method of coating probiotic microcapsule products is as follows: Add 3% stachyose and 8% morel enzymatic hydrolyzed powder to the fermented broth of Lactobacillus plantarum CGMCC NO.9405 fermented and cultivated in a fermenter , and then mixed with the wall material solution to obtain a mixed solution; adopt the method of ultrasonic vacuum spray drying, first-level vacuum drying, second-level inert protective gas plus vibrating fluidized bed drying, and microencapsulate the probiotics.

[0063] The method of ultrasonic spray drying adopts the method disclosed in the invention patent application 201110415081.8. Specifically, to turn on the ultrasonic spray drying equipment, after the vacuum pressure in the drying chamber is maintained at 2.5-3.0kPa, adjust the flow rate to 0-20mL / min, and the temperature in the drying chamb...

Embodiment 3

[0074] A probiotic microcapsule product is composed of wall material, Lactobacillus plantarum, stachyose, and hickory chick enzymolysis powder.

[0075] The method of coating probiotic microcapsule products is as follows: Add 5% stachyose and 5% morel enzymatic hydrolyzed powder to the fermentation broth of Lactobacillus plantarum CGMCC NO.9405 fermented and cultivated in a fermenter , and then mixed with the wall material solution to obtain a mixed solution; adopt the method of ultrasonic vacuum spray drying, first-level vacuum drying, second-level inert protective gas plus vibrating fluidized bed drying, and microencapsulate the probiotics.

[0076] The composition of the wall material solution is as follows: enzymatically hydrolyzed soybean protein isolate 10%, chitosan 0.5%, pullulan 0.5%, xanthan gum 0.2%, carrageenan 0.5%, glycerin 2%, trehalose 0.2%, The balance is distilled water; the above are weight and volume percentages.

[0077] Independently weigh various compon...

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Abstract

The invention provides a probiotic microcapsule and application thereof. The probiotic microcapsules are composed of wall material, plant lactobacillus, stachyose, and hickory chick enzymolysis powder. The method of coating the probiotic microcapsule product is as follows: add 3‑5% stachyose and 5‑8% hickory chick enzymatic hydrolyzed powder to the probiotic fermentation broth after fermentation and cultivation in a fermenter , and then the fermentation liquid is mixed with the wall material solution to obtain a mixed solution; the method of ultrasonic vacuum spray drying is adopted, the first level of vacuum drying, the second level of inert protective gas plus vibrating fluidized bed drying, and the probiotics are microencapsulated. The capsule of the present invention adopts modified soybean protein isolate, which has good enteric solubility. After the capsule reaches the intestinal tract, it can completely disintegrate within 1-1.5 hours to release probiotics, and rapidly proliferate to become the dominant flora, thereby inhibiting the growth of pathogenic bacteria And so on. The invention utilizes the hickory chick enzymolyzed powder, so that the product has the nutritional characteristics of the hickory chick, has good palatability, and improves the animal feeding effect.

Description

technical field [0001] The invention relates to a probiotic capsule product and its application, and belongs to the technical field of microbial feed additives. Background technique [0002] Lactic acid bacteria microecological feed additives are an important alternative to antibacterial drugs. Lactic acid bacteria are one of the outstanding representatives of beneficial bacteria in the normal intestinal flora, and have important physiological and health functions: they can antagonize pathogenic microorganisms, regulate the balance of animal digestive tract microflora: activate the immune system, enhance immunity, prevent various The occurrence of diseases and adverse reactions; inhibit the occurrence of tumors, protect the health of animals; synthesize nutrients, produce digestive enzymes, improve the activity of animal digestive enzymes, improve the metabolism of vitamins, neutralize enterotoxins, and reduce the harmful substances such as amines and ammonia. generate etc....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/18
CPCA23K10/12A23K10/14A23K10/18A23K10/30A23K20/147A23K20/163A23K40/30A23V2400/169
Inventor 王诚
Owner SHANDONG JIANYUAN BIOTECHNOLOGY CO LTD
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