Preparation method and application of molecularly imprinted polymer for extracting dencichine

An imprinted polymer and molecular imprinting technology, applied in the field of medicine, can solve the problems of shortening the coagulation time of mice, high price, low purity of Panax notoginseng, etc., and achieve the effects of reliable source of raw materials, simple regeneration method and strong selectivity.

Active Publication Date: 2015-06-17
BEIJING DAWN AEROSPACE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The water-soluble component of Panax notoginseng is a special amino acid isolated from the root of Panax notoginseng, which can shorten the coagulation time of mice and significantly increase the number of platelets, but there is no production of Panax notoginseng preparations yet and use
Mainly there are the following reasons: the template "notoginseng" is expensive, considering the cost and income, it is unreasonable to use the template "notanoginsin" to prepare molecularly imprinted polymers on a large scale. If general substances are used instead of notoginseng , there will be poor selectivity of molecularly imprinted polymers to notoginsin, and the purity of notoginsin obtained is very low. So far, there is no cheap substance to replace notoginsin, and the obtained molecularly imprinted polymers Strong selectivity, can be used for directional enrichment of notoginseng

Method used

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  • Preparation method and application of molecularly imprinted polymer for extracting dencichine
  • Preparation method and application of molecularly imprinted polymer for extracting dencichine
  • Preparation method and application of molecularly imprinted polymer for extracting dencichine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation of notoginseng pseudo-template molecularly imprinted polymers:

[0035] (1) material preparation

[0036] Each raw material component is formulated according to the following dosage ratio: pseudotemplate molecule phenylpyruvate, functional monomer 4-vinylpyridine 1.66g, crosslinking agent ethylene glycol dimethacrylate 16.12g, initiator azobisisobutyl Nitrile 0.13g, pore forming agent chloroform 45.34g;

[0037] (2) Preparation of polymers with template molecules

[0038] Add the prepared phenylpyruvate, 4-vinylpyridine, ethylene glycol dimethacrylate, azobisisobutyronitrile, and chloroform into the reaction kettle and mix evenly, ultrasonically degas for 15 minutes, and then fill with nitrogen to remove oxygen After sealing for 15 minutes, polymerize in a constant temperature water bath at 60°C for 24 hours to obtain a polymer with template molecules;

[0039] (3) Preparation of molecularly imprinted polymers that remove template molecules

[0040] Grin...

Embodiment 2

[0045] Preparation of notoginseng pseudo-template molecularly imprinted polymers:

[0046] (1) material preparation

[0047] The components of this example are formulated according to the following dosage ratio: template molecule DL-tyrosine 0.91g, functional monoacrylamide 1.83g, crosslinking agent N,N-dimethylbisacrylamide 24.00g, initiator azo Diisobutyronitrile 0.15g, solvent chloroform 50.00g.

[0048] (2) Preparation of polymers with template molecules

[0049] Add DL-tyrosine, acrylamide, N,N-dimethylbisacrylamide, azobisisobutyronitrile and chloroform prepared in step (1) into the reaction kettle and mix evenly, degas it by ultrasonic for 15 minutes, Then fill with nitrogen and remove oxygen for 15 minutes, seal and polymerize in a constant temperature water bath at 60°C for 24 hours to obtain a polymer with template molecules;

[0050] (3) Preparation of molecularly imprinted polymers that remove template molecules

[0051] Grinding the polymer prepared in step (2) ...

Embodiment 3

[0056] Preparation of notoginseng pseudo-template molecularly imprinted polymers:

[0057] (1) material preparation

[0058] The components of this embodiment are proportioned according to the following dosages: template molecule glycine-DL-leucine 0.85g, functional monomer methacrylic acid 1.66g, crosslinking agent divinylbenzene 21.10g, initiator (azobis Isovaleronitrile) 0.10g, solvent chloroform 47.00g.

[0059] (2) Preparation of polymers with template molecules

[0060] Add glycine-DL-leucine, methacrylic acid, divinylbenzene, azobisisovaleronitrile, and chloroform prepared in step (1) into the reaction kettle and mix evenly, ultrasonically degas for 15 minutes, and then fill with nitrogen to remove Oxygen for 15 minutes, sealed and polymerized in a constant temperature water bath at 60°C for 24 hours to obtain a polymer with template molecules; then grind it through a 200-mesh sieve to obtain molecularly imprinted polymer particles with template molecules notoginsin. ...

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Abstract

The invention discloses a preparation method and application of a molecularly imprinted polymer for extracting dencichine. The molecularly imprinted polymer of the dencichine is taken as a column chromatography filler and loaded in a column; then, a pseudo-ginseng root extract is applied to the column, and then a mixed solution of n-hexane / ethanol in a volume ratio of 10:1 is adopted for drip washing, and finally, the chromatographic column is eluted by use of alcohol and the effluent is the dencichine, wherein the alcohol refers to ethanol, isopropyl alcohol, n-propyl alcohol or n-butyl alcohol. The molecular imprinting technology is applied to extracting the dencichine in the pseudo-ginseng for the first time; compared with the traditional extraction method, the high-purity dencichine can be prepared quickly by virtue of the oriented enrichment effect of the molecular imprinting-solid-phase extraction column, and a raw material source is provided for the clinical pharmacological research of the dencichine.

Description

technical field [0001] The invention relates to a method in the field of pharmaceutical technology, in particular to a preparation method and application of a molecularly imprinted polymer for extracting notoginseng. Background technique [0002] The precious Chinese medicine "Sanqi" has the functions of promoting blood circulation and removing blood stasis, reducing swelling and pain, relaxing tendons and activating collaterals, among which it is famous for its hemostatic function. It is one of the raw materials of the famous Chinese medicine preparation "Yunnan Baiyao". "Compendium of Materia Medica" states that "Sanqi stops bleeding, dissipates blood and relieves pain", and Sanqi has the reputation of "stopping bleeding without leaving stasis, removing blood stasis without hurting the body", and it is actually an important medicine for regulating blood. Physicians of the past dynasties have used Panax notoginseng as the top grade to stop bleeding, and are widely used for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/56C08F220/06C08F226/06C08F222/14C08J9/28
Inventor 纪文华王晓黄璐琦高乾善刘伟耿岩玲
Owner BEIJING DAWN AEROSPACE BIO TECH
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