Method for detection of flavonoids in Artemisia baicalensis by high performance liquid chromatography

A technique of high performance liquid chromatography and Artemisia annua, applied in the field of detecting flavonoid components of Artemisia annua by high performance liquid chromatography, can solve problems such as no report on the detection method of Artemisia annua Handling simple effects

Active Publication Date: 2017-06-16
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there is no report on the detection method of the components of Artemisia annua

Method used

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  • Method for detection of flavonoids in Artemisia baicalensis by high performance liquid chromatography
  • Method for detection of flavonoids in Artemisia baicalensis by high performance liquid chromatography
  • Method for detection of flavonoids in Artemisia baicalensis by high performance liquid chromatography

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0030] Example 1. Detection of flavonoids in Artemisia annua growing in semi-mobile sand dunes

[0031] Test sample treatment: Collect fresh plant samples of Artemisia annua growing in semi-mobile sand dunes, dry in the shade, crush and pass through a 100-mesh sieve. Weigh 0.20 g of the sample, dissolve it in 20 mL of ethanol, and extract it ultrasonically for 30 min; take 1.25 mL in a 1.5 mL centrifuge tube, centrifuge for 5 min, and filter the supernatant through a microporous membrane (0.45 μm) to obtain liquid.

[0032] Liquid chromatographic conditions: Agilent-1100 high performance liquid chromatograph; GraceSmart RP18 reverse-phase chromatographic column (4.6mm×250mm, 5 μm). Mobile phase: 0.2% by mass acetic acid water (phase A) and methanol (phase B), the total flow rate is 0.6mL / min, and the detection wavelength is 275nm.

[0033] The volume ratio, detection sequence and retention time of the mobile phase: in order:

[0034] 0-5 min, the volume ratio of component B...

Embodiment 2

[0043] Example 2. Detection of flavonoids in Artemisia annua growing in the semi-arid Loess Plateau

[0044] Test sample treatment: Collect fresh plant samples of Artemisia annua growing in the semi-arid Loess Plateau, dry in the shade, crush and pass through a 100-mesh sieve. Weigh 0.20 g of the sample, dissolve it in 20 mL of ethanol, and extract it ultrasonically for 30 min; take 1.25 mL in a 1.5 mL centrifuge tube, centrifuge for 5 min, and filter the supernatant through a microporous membrane (0.45 μm) to obtain liquid.

[0045] Liquid chromatographic conditions: Agilent-1100 high performance liquid chromatograph; GraceSmart RP18 reverse-phase chromatographic column (4.6mm×250mm, 5 μm). Mobile phase: 0.2% by mass acetic acid water (phase A) and methanol (phase B), the total flow rate is 0.6mL / min, and the detection wavelength is 275nm.

[0046] Chromatographic method is with example 1.

[0047] Test results: See Table 3 for the flavonoid components of Artemisia annua g...

Embodiment 3

[0050] Example 3. Detection of flavonoids in different periods of Artemisia annua growing in a semi-mobile sand dune environment

[0051]Test sample treatment: Collect fresh plant samples of Artemisia baicalensis in different periods (July, August and September) in the environment of semi-mobile sand dunes, dry them in the shade, crush them and pass them through a 100-mesh sieve. Weigh 0.20 g of the sample, dissolve it in 20 mL of ethanol, and extract it ultrasonically for 30 min; take 1.25 mL in a 1.5 mL centrifuge tube, centrifuge for 5 min, and filter the supernatant through a microporous membrane (0.45 μm) to be the test solution .

[0052] Liquid chromatographic conditions: Agilent-1100 high performance liquid chromatograph; GraceSmart RP18 reverse-phase chromatographic column (4.6mm×250mm, 5 μm). Mobile phase: 0.2% by mass acetic acid water (phase A) and methanol (phase B), the total flow rate is 0.6mL / min, and the detection wavelength is 275nm.

[0053] Chromatographi...

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Abstract

The invention provides a method for detecting a flavone component and content thereof in roundhead wormwood by using a high performance liquid chromatography. According to the method, acetic acid water with mass percent of 0.2% and methanol are used as a mobile phase, the total flow speed of the mobile phase is 0.3-1.5mL / min and the detection wavelength is 275nm; and the method can be used for accurately and effectively analyzing and detecting types and content of the flavone components of the roundhead wormwood. The method has the advantages that the sample treatment is simple, the repeatability is good, the detection precision is high and the detection cost is low, and has great significances on deeply researching the stress resistance of the roundhead wormwood and scientifically and reasonably developing roundhead wormwood resources.

Description

technical field [0001] The invention relates to a method for detecting the types and contents of flavonoids in Artemisia baicalensis, in particular to a method for detecting flavonoids in Artemisia baicalensis by high performance liquid chromatography. Background technique [0002] White Artemisia Artemisia sphaerocephala Kraschen is a plant of the genus Artemisia in the family Asteraceae, also known as Artemisia chinensis, a semi-shrub, born in mobile sand dunes and semi-fixed sand dunes in deserts and semi-desert zones. Due to long-term living in the harsh environment of dry climate, strong sunshine, large temperature difference between day and night, and windy and sandy environment, Artemisia alba has formed its own unique adaptation mechanism. Studies have reported that a variety of flavonoids isolated from Artemisia annua have obvious antioxidant activity, can scavenge free radicals, and reduce the loss of plants in harsh environments. In addition, the flavonoids con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 刘权傅华南志标
Owner LANZHOU UNIVERSITY
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