A bacillus subtilis strain and applications thereof

A technology of Bacillus subtilis and strains, applied in the direction of bacteria, enzymes, biochemical equipment and methods, etc., can solve the problems of long production cycle and increased production cost of production cycle

Active Publication Date: 2015-07-08
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, most of the widely used fine strains are Trichoderma, Penicillium, Aspergillus niger and other fungi. Due to the long production cycle used by such fungi to produce cellulase, a large amount of toxic substances are also produced while producing enzymes. , the crude enzymes produced by its metabolism cannot be directly added to the feed, so technical procedures such as mem

Method used

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  • A bacillus subtilis strain and applications thereof
  • A bacillus subtilis strain and applications thereof
  • A bacillus subtilis strain and applications thereof

Examples

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Embodiment Construction

[0018] A Bacillus subtilis strain SB13 (Bacillus subtilis) of the present invention is obtained by culturing and enriching the rotten rice straw soil collected from the farmland around Mangdang Mountain, Nanping City, Fujian Province, and screening out endoglucanase and xylan. enzyme strain, and a complex solution containing endoglucanase and xylanase is prepared by the strain.

[0019] 1. Screening and isolation of the bacterial strain SB13

[0020] The soil containing rotten straw was collected from the farmland around Mangdang Mountain, Nanping City, Fujian Province, and dried naturally at room temperature for 7 days. After that, 5 g of the dried soil was weighed in a triangular flask equipped with 195 ml of enrichment medium, under the condition of 160 r / min Cultivate and enrich at a constant temperature at 30°C for 5 days to obtain the enriched bacterial solution; take 1g of the enriched bacterial solution for dilution, and the dilution factor is 10 -5 、10 -6 、10 -7 , ...

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Abstract

The invention provides a bacillus subtilis strain SB13 which is deposited in the China General Microbiological Culture Collection Center in February 28, 2014 and which has an accession number of CGMCC No.8867, and discloses a composite liquid prepared by the bacillus subtilis strain SB13 and containing endoglucanase and xylanase simultaneously, namely the bacillus subtilis strain SB13 can ferment and grow by adopting bean curd waste water as the only nutrition compound, and produce an enzyme solution containing the endoglucanase and the xylanase, thus providing a route of recovery and reutilization of the bean curd waste water, and providing a new fermentation bacterial source for production of endoglucanase and xylanase preparations in the feed processing industry.

Description

【Technical field】 [0001] The invention relates to a microorganism and its application, in particular to a bacillus subtilis strain, which can produce endoglucanase and xylanase simultaneously by utilizing tofu wastewater. 【Background technique】 [0002] In order to make full use of resources and save costs, substances with high cellulose and hemicellulose content, such as rice bran, wheat flour, cottonseed meal, straw powder, pasture, etc., are often used as raw materials to replace high-priced corn, soybeans, wheat and other substances; However, a high proportion of crude fiber diets not only cannot be directly absorbed and utilized by livestock and poultry, but also easily cause various problems such as diarrhea and slow growth of livestock and poultry. In order to improve feed quality, feed mills and farms have fully realized the importance of adding enzymes to feed, especially non-digestive enzymes such as cellulase and hemicellulase that cannot be synthesized by animals...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/42C12R1/125
CPCC12N9/244C12N9/248C12Y302/01006C12N1/205C12R2001/125
Inventor 官雪芳徐庆贤林斌钱蕾
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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