Helicobacter pylori transport culture medium as well as preparation method and application thereof

Abstract

The invention relates to the field of microbiology, and in particular relates to a helicobacter pylori transport culture medium as well as a preparation method and application thereof. The transport culture medium comprises agar and reducing agents and can be used for effectively ensuring a micro-aerophilic environment of helicobacter pylori, and thus the active state of a clinical sample can be stored as long as possible, the survival time of the clinical sample in a non-culture state can reach more than 48 hours, the long-distance transport of a specimen can be facilitated, and the transport culture medium is beneficial for hospitals and inspectors to flexibly arrange the specimen examination time. According to the helicobacter pylori transport culture medium disclosed by the invention, the active state of the sample can be stored for a long time, so that the helicobacter pylori transport culture medium has a very good promotion effect on subsequent culture, can prevent collected helicobacter pylori from being transformed into a non-culturable state, and ensures that the positive detection rate can be greatly improved; and compared with a method without using the transport culture medium in the past, the positive detection rate can be increased by not less than 40% by adopting the transport culture medium disclosed by the invention.

Description

technical field [0001] The invention relates to the field of microbiology, in particular to a Helicobacter pylori transport medium and its preparation and application. Background technique [0002] Helicobacter pylori (Hp) is a gram-negative microaerophilic bacterium. Warren and Marshall, winners of the Nobel Prize in Physiology and Medicine in 2005, isolated H. H. pylori infection is distributed globally. H. pylori is closely related to the occurrence of some upper gastrointestinal diseases. It is an important pathogenic factor of chronic gastritis and gastroduodenal ulcer. It is closely related to the occurrence of gastric cancer and gastric mucosa-associated lymphoid tissue lymphoma. The WHO has classified it as a causative agent. [0003] At this stage, clinical tests are mainly divided into two categories: invasive and non-invasive. The former includes bacterial culture and rapid urine enzyme test, and the latter includes urine breathing test and antibody detection. ...

AI Technical Summary

Problems solved by technology

Helicobacter pylori cannot grow and reproduce in the natural environment. It will take some time from the collection of samples to the actual start of culture and detection in clinical practice, and this period of time is crucial for the subsequent culture and reproduction of Helicobacter pylori. , even with the best culture medium, the positive detection rate will be greatly reduced

At present, there is still relatively little research on the transportation and storage conditions of specimens in China, or the attention is not high.

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