A kind of preparation method of high-purity trypsin

Abstract

A preparing method of high-purity trypsin is disclosed. The preparing method includes following steps: (1) dissolving a crude product, (2) inactivating viruses by a low-pH incubation method, (3) purifying by hydrophobic chromatography, (4) performing zymogen activation, (5) purifying by affinity chromatography, (6) desalting an eluate, (7) performing membrane filtration to remove the viruses and (8) performing vacuum freeze-drying to obtain the high-purity trypsin. According to the preparing method, the hydrophobic chromatography is adopted to purify the zymogen of the trypsin, the trypsin is purified by the affinity chromatography after the zymogen activation, a process is simple and convenient, and the high-purity high-titer trypsin can be obtained.

Description

technical field

[0001] The invention relates to the technical field of biochemical drug pharmacy, in particular to a preparation method of high-purity trypsin. Background technique

[0002] Trypsin is an important proteolytic enzyme secreted by animal pancreas, which can hydrolyze proteins into polypeptides or amino acids. It has selective hydrolysis of peptide chains of arginine and lysine, and is an endopeptidase. Trypsin can digest pus and necrotic tissue, so that normal tissue can secrete serum to remove foreign matter and promote the growth of new granulation. It has a wide range of applications, including: 1. Local edema, hematoma, abscess, etc. caused by various ulcers, inflammatory gangrene, traumatic injury, etc.; 2. Bronchitis, bronchial asthma, dry cough, etc. ); 3. All kinds of snake bites, etc.

[0003] In the prior art, the preparation of trypsin has been using multiple salting-out, crystallization and dialysis processes for a long time. This process has wea...