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Molecular marker method for detecting low abdominal fat percentage of Gaoyou ducks and application

A low abdominal fat rate and molecular marker technology, applied in the field of livestock and poultry molecular biology, can solve limited problems and achieve the effect of low abdominal fat rate

Inactive Publication Date: 2015-07-08
YANGZHOU UNIV
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Problems solved by technology

At present, although some progress has been made in the identification and application of duck fat deposition candidate genes, there are very few that can really be used to select low abdominal fat rate for breeding practice, so it is very necessary to find effective candidates that can affect duck fat deposition Genes and Molecular Markers

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  • Molecular marker method for detecting low abdominal fat percentage of Gaoyou ducks and application
  • Molecular marker method for detecting low abdominal fat percentage of Gaoyou ducks and application
  • Molecular marker method for detecting low abdominal fat percentage of Gaoyou ducks and application

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Embodiment 1

[0024] 1. Experimental materials

[0025] In the Gaoyou Duck Breeding Base, 280 Gaoyou ducks (half male and half female) were randomly selected from the breeding farm. The experimental ducks were reared routinely. During the breeding period, there were pools and activity venues. They were weighed on an empty stomach every morning and raised on the ground until the 10th. week. Blood was collected from the hindwing vein at 10 weeks old and preserved after anticoagulation. Genomic DNA was extracted by conventional phenol / chloroform extraction. Slaughter is carried out after the live weight is measured, and carcass performance indicators are measured. Slaughter measurements were carried out in accordance with the uniform standards of the National Poultry Breeding Committee, and the eviscerated weight and abdominal fat weight were measured, and the abdominal fat rate was calculated. Abdominal fat rate=abdominal fat weight / (whole eviscerated weight+abdominal fat weight).

[0026]...

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Abstract

The invention discloses a molecular marker method for detecting the low abdominal fat percentage of Gaoyou ducks. The molecular marker method comprises the following steps: by taking the whole-genome DNA of the Gaoyou ducks to be detected and containing VLDLR genes as a template and taking a primer pair P as primers, carrying out PCR amplification on the homology domain of the epidermal growth factor precursors of the VLDLR genes of the Gaoyou ducks, with an amplification length of 676bp, carrying out an agarose gel electrophoresis detection and then sequencing, and judging the polymorphism of the homology domain of the epidermal growth factor precursors of the VLDLR genes of the Gaoyou ducks according to a sequencing result; then carrying out an association analysis between the haplotype combination of the VLDLR genes of male ducks / female ducks and slaughter traits, and finding effective molecular marker information capable of influencing fat deposition and the abdominal fat percentage, and then culturing a high-quality Gaoyou meat duck strain with the low abdominal fat percentage.

Description

technical field [0001] The invention belongs to the technical field of molecular biology of livestock and poultry, and in particular relates to a molecular marker method related to duck belly fat rate for detecting low belly fat rate of Gaoyou duck. The molecular marker method can be applied to duck marker-assisted selective breeding. Background technique [0002] Gaoyou Duck is originally produced in Gaoyou, Jiangsu Province. It is a well-known fine breed of both egg and meat that is good at producing double-yolk eggs in my country, and has been included in the national livestock and poultry breed resources. However, Gaoyou Duck is relatively large in size and has high basic consumption. As a breed that can serve both eggs and meat, its egg production rate is lower than other breeds. Therefore, how to develop the traits of fast growth and low fat content is the first priority in breeding. The breeding of Gaoyou ducks has been carried out for more than 20 years, and the bree...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张汤杰赵南南王志强王聪
Owner YANGZHOU UNIV
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