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Microfluidic droplet chip device and method for cell migration analysis experiments

A cell migration and microfluidic technology, applied in the field of microfluidic analysis, can solve problems in the initial stage and achieve accurate cell counting

Active Publication Date: 2017-03-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Droplet microfluidic technology has the characteristics of micro volume, high efficiency and high throughput, but its application in cell biology is still in its infancy

Method used

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  • Microfluidic droplet chip device and method for cell migration analysis experiments
  • Microfluidic droplet chip device and method for cell migration analysis experiments
  • Microfluidic droplet chip device and method for cell migration analysis experiments

Examples

Experimental program
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Effect test

Embodiment 1

[0060] Microfluidic Droplet Chip Devices for Studying Competitive Cell Migration, see figure 1 with Figure 2a , figure 1 is the longitudinal section view of the device, Figure 2a is the top view of the device, Figure 2b for Figure 2a A partial enlargement of the . The entire chip is circular, placed in a transparent plastic petri dish with a diameter of 6 cm, and fluorine oil (immiscible with aqueous solution, and has a certain degree of air permeability) is added to immerse the chip as an anti-liquid evaporation component 6, and an acrylic ring with a thickness of 7 mm Placed on the chip as a compact 5 to prevent the chip from floating in the oil phase. The porous membrane 1 is a translucent polycarbonate membrane (PC) with a pore diameter of 8 microns and a thickness of 20 microns. A polydimethylsiloxane (PDMS) chip containing an array of through holes is used as the droplet position control component 3 and the droplet position control component 4 to be attached to...

Embodiment 2

[0065] Microfluidic Droplet Chips for Studying Cell Chemotaxis and Migration, see Figure 4a , Figure 4b with Figure 5a , Figure 5b , Figure 4a For the device of spotting liquid droplets for three-dimensional chemotaxis and migration experiments of cells, Figure 4b for Figure 4a A partial enlargement of the Figure 5a Top view of the setup for spotting droplets for 3D chemotaxis and migration experiments of cells, Figure 5b for Figure 5a A partial enlargement of the . The entire chip is circular and placed in a transparent plastic culture dish with a diameter of 6 cm. The chip is immersed in fluorine oil as an anti-evaporation component 6, and an acrylic ring with a thickness of 7 mm is used as a pressing block 5 to prevent the chip from being in the oil phase. float in. The porous membrane 1 is a PC membrane with a pore diameter of 8 microns and a thickness of 20 microns. A PDMS chip containing an array of through holes is used as the droplet position contro...

Embodiment 3

[0070] A microfluidic liquid droplet chip device for cell migration under the action of multiple cells, the structural diagram of the device can be found in Figure 8a , Figure 8b with Figure 9a with Figure 9b , Figure 8a is the longitudinal section view of the device, Figure 8b for Figure 8a A partial enlargement of the Figure 9a is the top view of the device, Figure 9b for Figure 9a A partial enlargement of the . The entire chip is circular and placed in a transparent plastic culture dish with a diameter of 6 cm. The chip is immersed in fluorine oil as an anti-evaporation component 6, and an acrylic ring with a thickness of 7 mm is used as a pressing block 5 to prevent the chip from being in the oil phase. float in. The porous membrane 1 is a PC membrane with a pore diameter of 8 microns and a thickness of 20 microns. A PDMS chip containing an array of through holes is used as the droplet position control component 3 and the droplet position control compon...

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Abstract

The invention discloses a microfluidic liquid drop chip device for cell migration analysis experiment. The microfluidic liquid drop chip device comprises a porous membrane, a porous membrane supporting assembly and a liquid evaporation prevention assembly, wherein the porous membrane is used for bearing liquid drops, the porous membrane supporting assembly is used for ensuring that the porous membrane is at a spreading state, and the liquid evaporation prevention assembly surrounds the periphery of the porous membrane and is used for preventing liquid evaporation. Meanwhile, the invention discloses a method for cell migration by using the device. The microfluidic liquid drop chip device combines the advantages of a microfluidic chip and a liquid drop technology; the flexible miniature cell experimental device is constructed by using the change of relative locations of liquid drops on the upper surface and lower surface of the porous membrane and is used for research of multiple cell migration modes, such as competitive cell migration, cell chemotactic migration and multi-cell concurrent migration. The microfluidic liquid drop chip device is suitable for research on pathological physiology mechanisms of high throughout drug screening and cell migration behaviors and research on influence of peripheral cells or matters on cell migration behaviors.

Description

technical field [0001] The field of the invention relates to the field of microfluidic analysis, in particular to a microfluidic droplet chip device used for cell migration analysis experiments and a method for using the device to perform cell migration experiments. Background technique [0002] Cell migration is closely related to a variety of physiological and pathological phenomena. The proliferation of primary cancer cells, the immune response of immune cells, and the embryonic development process all involve cell migration. It can be seen that cell migration is an important content in the study of pathological and physiological issues. Traditional methods for studying cell migration include scratch method, agarose plate method, Boyden chamber and other methods, but the traditional method consumes a lot of reagents, has low throughput, and the cell count is not accurate enough. It is often difficult to observe in real time, and it is difficult to simulate a complex enviro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12Q1/02
Inventor 方群马妍
Owner ZHEJIANG UNIV
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