Micro-fluidic chip for monitoring virus electrical impedance in real time

Abstract

The invention discloses a micro-fluidic chip for monitoring virus electrical impedance in real time, the micro-fluidic chip comprises a chip substrate, a shielding electrode ring, a sensor array and a shielding electrode, the upper surface of the chip substrate comprises a liquid storage tank, a shielding electrode ring groove and a circular electrode groove, the shielding electrode ring groove and the circular electrode groove are embedded in the liquid storage tank, and the shielding electrode ring groove and the circular electrode groove are embedded in the shielding electrode ring groove. The lower surface of the chip base body comprises a strip-shaped electrode groove and a shielding electrode groove, the sensor array is formed by connecting a round electrode and a strip-shaped electrode, enrichment and real-time monitoring of virus infected cells are achieved, the original long-time-consuming and multi-step virus detection process is shortened to several minutes, the detection efficiency is improved through a single step, the cells infected by viruses are monitored in real time, death of the viruses or the cells in the cell transferring or processing process in a traditional sampling method is avoided, the virus detection accuracy is greatly improved, meanwhile, the six liquid storage tanks are arranged, multiple detection solutions can be injected at the same time for virus detection, and the virus detection reliability is improved.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a microfluidic chip for real-time monitoring of virus electrical impedance, belonging to the technical field of microfluidic chips. Background technique [0002] The current coronavirus detection methods mainly include nucleic acid detection and antibody detection. The nucleic acid detection process includes multiple steps such as specimen processing, nucleic acid extraction, and PCR detection. The average detection time is long, requiring 2-3 hours. It directly detects the viral nucleic acid in the collected specimens. It has strong specificity and relatively high sensitivity. It is the main detection method at present. Antibody detection is to detect the antibody level in human blood. There is a detection window period and can be used for Auxiliary diagnosis of cases with negative nucleic acid tests can also be used to screen cases, but it cannot replace nucleic acid testing methods. Gen...

AI Technical Summary

Problems solved by technology

However, BIS has two problems in the detection process of coronavirus: one is that the concentration of coronavirus-infected cells in the sample affects the measurement results of BIS, thereby affecting the analysis of sample components; Use ultra-high frequency excitation signals to penetrate the cell membrane to detect whether it is parasitized by viruses. However, ultra-high frequency BIS has extremely high requirements for detection chips and peripheral circuits

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