Method for preparing cervical cancer resistant polypeptide from tuna dark meat protein

A technology of tuna and cervical cancer, applied in the field of preparation of fish functional peptides, to achieve the effect of safety, non-toxic and side effects

Active Publication Date: 2015-07-15
北京姿美堂生物技术股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The applicant's research found that the preparation of anti-cervical cancer polypeptides using enzymatic hydrolysis technology using the dark meat of tuna as raw materials is still in a blank stage, and there is no report on the preparation of highly active anti-cervical cancer polypeptides using enzymatic hydrolysis products as materials.

Method used

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  • Method for preparing cervical cancer resistant polypeptide from tuna dark meat protein
  • Method for preparing cervical cancer resistant polypeptide from tuna dark meat protein
  • Method for preparing cervical cancer resistant polypeptide from tuna dark meat protein

Examples

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Embodiment

[0025] 1) Pretreatment of dark tuna meat: Tuna dark flesh was homogenized, heated to 95°C and kept warm for 10 min, then cooled to room temperature, added isopropanol according to the ratio of solid to liquid 1g: 5mL, degreased at room temperature for 24 h, and then centrifuged at 4°C, 10000 rpm for 20 min to remove isopropanol. Propanol collects the solid matter of the dark meat of the defatted tuna, which is the protein of the dark meat of the defatted tuna;

[0026] 2) Enzymatic hydrolysis of dark meat protein from defatted tuna: Using defatted tuna dark meat protein as raw material, add Gly-NaOH buffer solution (0.05 mol / L, pH 9.5) at a solid-to-liquid ratio of 1 g : 25 mL to obtain a mixed solution; raise the temperature of the mixed solution to 50 °C and preheat for 10 min , add protease according to 2% of the weight of the dark meat of defatted tuna, and the enzymolysis temperature is 50°C. After 4 hours of enzymolysis, the solution is heated to 95°C and kept at this ...

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Abstract

The invention discloses a method for preparing a cervical cancer resistant polypeptide from a tuna dark meat protein. The cervical cancer resistant polypeptide Gln-Tyr-Asp-Glu-Tyr-Trp is prepared by using tuna dark meat as a raw material and carrying out degreasing, enzymolysis with alkaline protease, ultrafiltration, gel filtration chromatography, cell membrane chromatography purification and reversed phase high performance liquid chromatography purification. The polypeptide has outstanding inhibiting effects on proliferation of cells for a human cervical cancer cell strain HeLa, has IC50 of 0.16mg / mL and can be used for preparing drugs for preventing and treating cervical cancers.

Description

technical field [0001] The invention relates to a preparation method of a fish functional polypeptide, in particular to a preparation method of a tuna dark meat protein anti-cervical cancer polypeptide. Background technique [0002] Tuna is an oceanic fish, and it is a key target fish species for the development of pelagic fisheries in the world. It is also recommended by the International Society of Nutrition as one of the world's three most nutritious fish. According to the statistics of the Food and Agriculture Organization of the United Nations, the total output of oceanic fisheries in the world is 8.5 million tons, of which the output of tuna exceeds 6 million tons, accounting for more than 70% of the total output of high seas fisheries. A large amount of dark flesh is produced during tuna processing, accounting for about 11% of the raw material. Existing studies have shown that the dark meat of tuna is rich in essential amino acids, comprehensive, easy to digest and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K7/06C07K1/36C07K1/34C07K1/16A61P35/00
Inventor 迟长凤王斌赵玉勤孙坤来
Owner 北京姿美堂生物技术股份有限公司
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