A kind of targeted mesoporous molecular imaging probe and preparation method thereof

Abstract

A targeted mesoporous molecular imaging probe and a preparation method thereof, the targeted mesoporous molecular imaging probe consists of a surface plasmon resonance core layer at least comprising gold nanorods I, gold nanorods II, and substrate nanoparticles, and a fluorescent Molecules, thiol biomolecules, and polystyrene sulfonate joints are composed of two outer cladding layers, wherein the gold nanorods 1 and 2 are conjugated to the base nanoparticles to form a surface plasmon resonance core layer , the distance between the gold nanorod 1 and the gold nanorod 2 satisfies that the gold nanorod 1 and the gold nanorod 2 are combined with the thiol biomolecules outside the core layer, the fluorescent molecules are combined with the thiol biomolecules through the amide covalent bond, and the polystyrenesulfonic acid The salt is directly bridged to the substrate. The invention also includes the preparation method of the targeting mesoporous molecular imaging probe. The present invention can be blue-shifted or red-shifted by surface excitation, which can match the luminous characteristics of fluorescent tissue, so that the luminous intensity can be increased by 8 to 10 orders of magnitude. The present invention has very high fluorescent characteristics and can be used for the diagnosis of tumor fluorescence imaging, especially Tumor detection and diagnosis in dark field deep in tissue cells.

Description

technical field [0001] The invention relates to a targeting mesoporous molecular imaging probe and a preparation method thereof, in particular to a targeting mesoporous molecular imaging probe based on surface plasmon resonance gold nanorods and a preparation method thereof. Background technique [0002] Visible light imaging has many advantages over nuclear magnetic resonance imaging, such as easy operation, intuitive results, fast measurement, no radiation to the human body, low environmental requirements, and low cost. Therefore, the application of visible light imaging technology to clinical medical diagnosis has become the current An important branch of medical imaging. [0003] At present, one of the main reasons limiting the clinical application of visible light imaging technology is the lack of suitable fluorescent probes for human body. At the same time, high contrast is also the main technical bottleneck that plagues fluorescent probes. For example, the prior art ...

AI Technical Summary

Problems solved by technology

Although the above-mentioned fluorescent probes based on gold nanomaterials in the prior art have solved the problems of safety and stability well, they all have a single luminescence wavelength of nanocrystals, which cannot be adjusted to suit the luminescence wavelength of fluorescent tissues, and provide high Problems with Brightness Fluorescence Imaging

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