Method for rapidly obtaining regenerated plants of fiber hemps

A technology for regenerating plants and hemp, which is applied in the field of quickly obtaining regenerated plants of hemp for fiber, can solve the problems of long cultivation period, etc., and achieve the effect of convenient material collection, uniform growth potential and short period

Active Publication Date: 2015-07-22
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

In the Chinese patent application with the application number 200610088353.7, the seeds are sterilized and then inoculated in the culture medium. The shoot tip explants can be obtained after 20 days, and the lateral buds can be obtained after 30 days. The cultivation period is longer

Method used

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  • Method for rapidly obtaining regenerated plants of fiber hemps
  • Method for rapidly obtaining regenerated plants of fiber hemps
  • Method for rapidly obtaining regenerated plants of fiber hemps

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Experimental program
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Embodiment 1

[0026] The method for rapidly obtaining fiber hemp regeneration plants of the present embodiment comprises the following steps:

[0027] (1) Aseptically treat and sow hemp seeds for fiber to obtain sterile seedlings;

[0028] See Cheng Chaohua, Li Yujun, Zhao Lining, Tang Qing, Zang Consolidation, Research on Obtaining Sterile Cannabis Seedlings by Triple Treatment [J]. Chinese Hemp Industry Science. 2011(01);

[0029] (2) On the ultra-clean workbench, tear off the cotyledons from the 3-day-old sterile seedlings (it is not suitable to cut off, otherwise it will affect the regeneration frequency), cut off the leaf tips, leave 1 / 2 cotyledons, and place them upright Adventitious buds were cultured on the bud induction medium containing thiadizuron (TDZ) and α-naphthylacetic acid (NAA); - The concentration of naphthalene acetic acid is 0.05mg / L, additional sucrose 30g / L, agar 6.5g / L, pH5.8, light intensity 2300lx, light time 16h / d, temperature 24±2°C, culture time is 20 days;

...

Embodiment 2

[0034] Select 2 days, 4 days, 5 days, 6 days of sterile seedlings respectively, and tear off the cotyledons for processing. Other processing steps are the same as in Example 1. See Table 1 for the results of regeneration frequency using explants of different seedling ages from 2 to 6 days. It can be seen that it is more suitable to adopt 2-day and 3-day seedling-age explants.

[0035] Table 1 Regeneration frequency of explants at different seedling ages

[0036] Seedling age of cotyledons (days) Regeneration frequency (%) 2 47.8±5.1 3 46.7±5.7 4 36.7±4.9 5 22.2±3.5 6 11.9±3.7

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Abstract

The invention provides a method for rapidly obtaining regenerated plants of fiber hemps. The method comprises the following steps: (1) carrying out sterile treatment on seeds of the fiber hemps and sowing seeds to obtain sterile seedlings; (2) tearing cotyledons from the sterile seedlings with the seedling ages of 2-3 days; cutting off leaf tips and putting into a bud induction culture medium containing thidiazuron and alpha-naphthylacetic acid to be cultured; (3) after culturing by the step (2) for 18-24 days, cutting off adventitious buds when the adventitious buds stretch to be 1cm-2cm high; transferring the adventitious buds into a rooting culture medium containing indolebutyric acid to be cultured and rooted, wherein the culture time is 35-42 days; and obtaining rooted seedlings. With the adoption of the method, explants are convenient and rapid to take, the period is short, the induction regeneration rate is relatively high and cotyledon explants can be obtained by only 3 days; and strong regenerated cluster buds are obtained by only 18-24 days; and seeds can be sowed at any time to obtain the explants, and the process is not limited by factors including material taking seasons, a disinfection process and the like, so that the method is suitable for hemp genetic transformation researches.

Description

technical field [0001] The invention relates to a method for rapidly obtaining regenerated plants of hemp for fiber. Background technique [0002] marijuana( Cannabis Sativa L.) is an annual herbaceous plant of the genus Cannabis in the family Cannabinaceae, and has a history of planting and utilization in my country for thousands of years. Hemp is full of treasures. It can not only produce high-quality textile fibers, but also has great application value in food, medicine and building materials industries. Cannabis is divided into three types according to its tetrahydrocannabinol (THC) content: drug cannabis, intermediate cannabis and fiber cannabis. my country is a big country in fiber cannabis cultivation, with the planting area accounting for about 30% of the world. [0003] Since many countries banned the cultivation of all cannabis in the last century, many precious germplasm resources were lost, and the research on cannabis lags far behind other economic crops. Mor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 程超华臧巩固赵立宁高春生唐蜻李育君范志芳
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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