TLC method for identification of Eucommia ulmoides and preparations containing eucommia and application of potassium bismuth iodide solution

A technology of thin-layer identification and bismuth potassium iodide, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inconvenient detection of preparations, many impurities spots, unclear color development, etc., and achieve strong specificity and low cost , the effect of less impurity interference

Active Publication Date: 2016-08-24
HUNAN HANSEN PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The invention solves the technical problems that the detection of the preparation containing Eucommia is not convenient enough, the color development is not clear, there are many impurity spots, and the reproducibility is poor

Method used

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  • TLC method for identification of Eucommia ulmoides and preparations containing eucommia and application of potassium bismuth iodide solution
  • TLC method for identification of Eucommia ulmoides and preparations containing eucommia and application of potassium bismuth iodide solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]Take 3g of the content powder of the blue-red capsule, add 20ml of methanol, ultrasonicate for 30 minutes, filter, the filtrate is concentrated to less than 3ml, transfer to a graduated centrifuge tube, and dilute to 3ml, shake well, stand or centrifuge, Take 1ml of the supernatant, put it on a neutral alumina SPE column (1g / 3ml), elute with 30ml of 20% ethanol solution, collect the eluate, evaporate to dryness, add 1ml of methanol to the residue to dissolve, and use it as the test solution; Get Eucommia reference drug powder 2g, make Eucommia reference drug solution with the preparation method of need testing solution; Get pinoresinol diglucoside as reference substance, add methanol to make 1ml solution containing 1mg, as reference substance solution; Preparation process, be mixed with the negative sample of blue-red capsule not containing Eucommia, and make negative test solution by the preparation method of above-mentioned need test solution; Test according to thin-lay...

Embodiment 2

[0036] Take blue-red capsules and operate according to the method of Example 1, except that the developing agent is: the lower layer solution of chloroform-methanol-water (3:1:0.1). In the chromatogram of the test product, there are spots of the same color at the positions corresponding to the chromatogram of the reference substance and the reference medicinal material.

Embodiment 3

[0038] Take blue-red capsules and operate according to the method of Example 1, except that the developing agent is the lower layer solution of toluene-ethyl acetate-methanol-water (5:3:2.5:2.5). In the chromatogram of the test product, there are spots of the same color at the positions corresponding to the chromatogram of the reference substance and the reference medicinal material.

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Abstract

The present invention relates to the TLC identification method of eucommia ulmoides and preparations containing eucommia and the application of bismuth potassium iodide solution. The TLC identification method of eucommia ulmoides comprises the following steps: (1) making the test sample and the standard product of eucommia ulmoides into test samples respectively solution and contrast solution; (2) need testing solution and contrast solution are spotted respectively on the thin-layer plate, develop with developing agent; (3) thin-layer plate is taken out after developing, dry, and spray chromogenic agent bismuth iodide Potassium solution, air-dried or oven-dried, and placed until the spots develop clear color, so as to realize TLC identification of Eucommia ulmoides. The bismuth potassium iodide solution selected in the present invention is particularly suitable for color development of the eucommia medicinal material and the eucommia-containing preparation, has the advantages of good reproducibility, strong specificity, simplicity and practicality, and can effectively control the quality of the eucommia medicinal material and the eucommia-containing preparation.

Description

technical field [0001] The invention relates to a TLC identification method for eucommia ulmoides and preparations containing eucommia ulmoides and the application of bismuth potassium iodide solution, which belongs to the field of thin layer chromatography of traditional Chinese medicinal materials. Background technique [0002] Blue red capsule is composed of blue thorn head, Eucommia ulmoides, safflower and other ingredients. It has the functions of promoting blood circulation and dispersing blood stasis, reducing swelling and pain, rejuvenating tendons and bones, etc. It is used to treat soft tissue contusion, crush injury, joint sprain, open wound, limb Traumatology good medicine for fractures, etc. Eucommia ulmoides is the dry bark of Eucommia ulmoides Oliv, a plant of the family Eucommia. Its processed products are divided into Eucommia ulmoides Oliv and salt Eucommia. Tocolysis and other effects. The medicinal parts of the Eucommia plant include Eucommia bark and E...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
Inventor 刘令安杨华谢金华郭元满谭泽云胡宇飞彭媛
Owner HUNAN HANSEN PHARMACEUTICAL CO LTD
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