Unlock instant, AI-driven research and patent intelligence for your innovation.

Isoflavones contained in tobacco and its preparation method and application

A kind of compound, technology of isoflavone, application in antioxidant and anti-HIV-1, isoflavone compound and preparation field thereof

Active Publication Date: 2017-11-24
CHINA TOBACCO YUNNAN IND
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention isolates a new isoflavone compound with anti-oxidation and anti-HIV-1 activity from tobacco, which has not been reported so far

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Isoflavones contained in tobacco and its preparation method and application
  • Isoflavones contained in tobacco and its preparation method and application
  • Isoflavones contained in tobacco and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] -Preparation of the compound

[0028] The tobacco sample was collected in Yuxi, Yunnan, and the variety was Yunyan-85. Sample 2.2kg of tobacco sample and crush it to 30 meshes, extract 3 times with 70% acetone ultrasonically, combine the extracts, filter, concentrate the extracts under reduced pressure to 1 / 6 of the original volume of the extracts, filter the precipitate after standing The filtrate was extracted with ethyl acetate three times, the ethyl acetate extracts were combined and concentrated into an extract to obtain 42.8 g of extract.

[0029] The extract is dissolved with appropriate amount of chloroform and mixed with 80g crude silica gel (80-100 mesh), 1.5kg silica gel (160-200 mesh) is packed in a column for silica gel column chromatography, the volume ratio of chloroform:acetone (1:0→0: 1) Gradient elution, TLC monitoring and merging the same parts to obtain 8 parts (pure chloroform, chloroform-acetone 20:1, chloroform-acetone 9:1, chloroform-acetone 8:2, chl...

Embodiment 2

[0031] -Preparation of the compound

[0032] The tobacco sample was collected in Yuxi, Yunnan, and the variety was Yunyan-85. Sample 2.2kg of tobacco sample and crush it to 30 mesh, extract 3 times with 70% acetone ultrasonically, combine the extracts, filter, concentrate the extracts under reduced pressure to 1 / 4 of the original volume of the extracts, filter the precipitate after standing The filtrate was extracted 3 times with ethyl acetate, and the ethyl acetate extracts were combined and concentrated into an extract to obtain 42.7 g of extract.

[0033] The extract is dissolved with appropriate amount of chloroform and mixed with 80g crude silica gel (80-100 mesh), 1.5kg silica gel (160-200 mesh) is packed in a column for silica gel column chromatography, the volume ratio of chloroform:acetone (1:0→0: 1) Gradient elution, TLC monitoring and merging the same parts to obtain 8 parts (pure chloroform, chloroform-acetone 20:1, chloroform-acetone 9:1, chloroform-acetone 8:2, chlor...

Embodiment 3

[0035] -Preparation of the compound

[0036] The tobacco sample was collected in Yuxi, Yunnan, and the variety was Yunyan-85. Sample 2.2kg of tobacco sample and crush it to 30 meshes, extract 3 times with 70% acetone ultrasonically, combine the extracts, filter, concentrate the extracts under reduced pressure to 1 / 5 of the original volume of the extracts, filter the precipitate after standing The filtrate was extracted 3 times with ethyl acetate, and the ethyl acetate extracts were combined and concentrated into an extract to obtain 42.9 g of extract.

[0037] The extract is dissolved with appropriate amount of chloroform and mixed with 80g crude silica gel (80-100 mesh), 1.5kg silica gel (160-200 mesh) is packed in a column for silica gel column chromatography, the volume ratio of chloroform:acetone (1:0→0: 1) Gradient elution, TLC monitoring and merging the same parts to obtain 8 parts (pure chloroform, chloroform-acetone 20:1, chloroform-acetone 9:1, chloroform-acetone 8:2, chl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses an isoflavone compound shown as formula (I) contained in tobacco and a preparation method and application thereof. The method is as below: crushing a tobacco sample, conducting ultrasound extraction with 70% acetone 3 times, merging the extracts, filtering, conducting reduced pressure concentration on the filtrate to1 / 4-1 / 6 volume of the extract, standing, filtering the precipitate, and extracting with ethyl acetate ester three times to obtain an ethyl acetate extract liquid; and concentrating the extract into an extract, primarily separating the extract by silica gel column chromatography, then further separating the extract by high performance liquid preparative chromatography, so as to obtain the desired isoflavone compound. Antioxidant activity and anti-HIV-1 activity screening is conducted on the compound, and the experimental results show that the compound has strong antioxidant and anti-HIV-1 activity.

Description

Technical field [0001] The present invention belongs to the field of tobacco chemistry. More specifically, the present invention relates to a new isoflavone compound contained in tobacco and a preparation method thereof. At the same time, it also relates to its application in anti-oxidation and anti-HIV-1. Background technique [0002] Tobacco is one of the most chemical substances in various plants known to humans. After decades of research, people have identified more than 3,000 monomeric chemical substances from tobacco, and there are still many components that have not yet been identified. come out. In addition to being mainly used for cigarette production, tobacco can also extract a variety of valuable chemical components; therefore, in addition to being consumed as cigarettes, it is of great significance to strengthen the comprehensive utilization of tobacco and its waste. [0003] Flavonoids are widely found in plants in nature and belong to the secondary metabolites of pla...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/36C07D311/40A61P39/06A61P31/18
CPCC07D311/36C07D311/40
Inventor 申钦鹏刘春波杨光宇张涛张凤梅何沛司晓喜苏钟璧刘志华缪明明
Owner CHINA TOBACCO YUNNAN IND