Apolygus lucorum soluble trehalase (AlTre-1) monoclonal antibody as well as preparation method and application of thereof
A monoclonal antibody, chlorotic bug technology, applied in anti-enzyme immunoglobulins, measuring devices, instruments, etc., can solve problems such as differences in inhibitory effects and decline in pesticide use effects, and achieve high-sensitivity effects.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] Example 1 Obtaining of the monoclonal antibody of Lygus chlorophylla AlTre-1
[0028]Antigen treatment is carried out first: the total amount of purified recombinant AlTre-1 protein is about 2 mg, and the doses of the primary, secondary, third and fourth immunizations are all 100 μg per mouse; the primary immunization antigen is protein antigen and an equal volume of Freund’s complete Mix and emulsify the agent; the second, third and fourth immune antigens are protein antigens mixed with an equal volume of Freund's incomplete adjuvant and emulsified. Then animal immunization: healthy 6-8 week-old female BALB / c mice were immunized by subcutaneous multi-point injection for a total of 4 times; the first immunization antigen was Freund's complete adjuvant and protein antigen; the second time after an interval of 21 days immunity. The third immunization antigen was used for the third immunization after an interval of 35 days; the fourth immunization antigen was used for the...
Embodiment 2
[0041] Example 2: Western blot detection of Lygus chlorophylla AlTre-1 monoclonal antibody
[0042] Take the AlTre-1 recombinant protein and load it according to the gradient. After loading the sample, run the polyacrylamide gel at 75V to finish the stacking gel, and then increase the voltage to 110V until the end of electrophoresis. After the electrophoresis, the gel was removed and transferred to the membrane at a constant voltage of 100V for 1.5 hours. After electroporation, remove the membrane and wash it with PBS 4 times, 5 minutes each time. Then place it at 37°C in 5% non-fat dry milk blocking solution for 1 hour. The primary antibody was diluted with blocking solution, and the membrane was reacted in the primary antibody diluent at 37°C for 1 hour. Wash the membrane 4 times, 5 minutes each time, dilute the secondary antibody with blocking solution containing 5% milk. The membrane was reacted in the secondary antibody at 37°C for 1 hour. Washed membrane ECL develop...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com