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Novel production method of lipase

A lipase and yield technology, applied in the field of lipase production, can solve the problems of large energy consumption, environmental pollution, and no economic value, and achieve the effects of improving the environment, reducing industrial costs, and turning waste into valuable applications.

Inactive Publication Date: 2015-08-12
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] A large amount of wastewater containing petroleum hydrocarbons will be produced in the process of petroleum production and processing. Various methods such as physics, chemistry and biology are used to treat oily wastewater. Among them, activated sludge method or contact oxidation method is often used in biological treatment processes. These processes When treating wastewater containing petroleum hydrocarbons, a large amount of energy is consumed, and at the same time, a large amount of aromatic hydrocarbons are volatilized, causing environmental pollution, and no appropriate economic value is generated.

Method used

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Experimental program
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specific Embodiment approach

[0010] The following is a detailed description of this patent in conjunction with specific embodiments.

[0011] (1) Obtain lipase-producing bacteria that utilize petroleum hydrocarbons: add 1 gram of oil-containing soil to 100 mL of inorganic salt medium, culture at 37°C for 7 days, transfer once, and repeat 7 times to obtain bacteria that can degrade petroleum hydrocarbons ability of bacteria. Subsequently, the lipase yield of the obtained bacterial strains was detected, and strains with high lipase yield were screened.

[0012] (2) Utilize petroleum-containing hydrocarbon wastewater to cultivate bacteria to produce lipase: the high-efficiency oilophilic bacteria obtained above is added by 5% inoculum to the oil production wastewater that petroleum hydrocarbon content is 100mg / L, from petroleum-containing hydrocarbon wastewater, lipase is separated, and Detect its content and activity.

[0013] (3) Ammonium sulfate precipitation method and ion exchange chromatography were ...

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Abstract

The invention discloses a novel method for preparing lipase from wastewater containing petroleum hydrocarbons. In the production method, a LB culture medium is adopted to breed bacteria capable of degrading the petroleum hydrocarbons; then the bacteria with 1% of the inoculant amount are domesticated in an inorganic salt culture medium containing 0.5% of crude oil for three times (7 days each time); then the domesticated bacteria is inoculated into wastewater containing petroleum hydrocarbons with a concentration of 100 mg / L with respect to an inoculant amount of 5%; after 24-hour fermentation, the activity of lipase in wastewater reaches 10 U / mL, and the lipase content reaches 25 gram / ton.

Description

Technical field: [0001] The invention relates to a new method for producing lipase by cultivating bacteria in waste water containing petroleum hydrocarbons. Background technique: [0002] Lipase (lipase, triacylglycreol acylhydrolases, EC 3.1.1.3), triacylglycerol acyl hydrolase, is one of the most important enzymes in industrial enzyme preparations, which can catalyze the hydrolysis of natural substrate oil at the oil-water interface to generate fatty acids, glycerol and At the same time, lipase can also catalyze various chemical reactions such as transesterification, lipid transfer, lipid synthesis, alcoholysis, acidolysis, ammonolysis, etc., and has high catalytic efficiency and simple catalytic conditions. Therefore, it is widely used It is used in industries such as detergent, leather, oil processing, paper making, and daily chemicals. [0003] A large amount of wastewater containing petroleum hydrocarbons will be produced in the process of petroleum production and pro...

Claims

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Application Information

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IPC IPC(8): C12N9/20
CPCC12N9/20C12Y301/01003
Inventor 罗明志闫筱邓林红邱国华周欢张治国石晓灏刘杨王悦
Owner CHANGZHOU UNIV