Novel production method of lipase

Abstract

The invention discloses a novel method for preparing lipase from wastewater containing petroleum hydrocarbons. In the production method, a LB culture medium is adopted to breed bacteria capable of degrading the petroleum hydrocarbons; then the bacteria with 1% of the inoculant amount are domesticated in an inorganic salt culture medium containing 0.5% of crude oil for three times (7 days each time); then the domesticated bacteria is inoculated into wastewater containing petroleum hydrocarbons with a concentration of 100 mg / L with respect to an inoculant amount of 5%; after 24-hour fermentation, the activity of lipase in wastewater reaches 10 U / mL, and the lipase content reaches 25 gram / ton.

Description

Technical field:

[0001] The invention relates to a new method for producing lipase by cultivating bacteria in waste water containing petroleum hydrocarbons. Background technique:

[0002] Lipase (lipase, triacylglycreol acylhydrolases, EC 3.1.1.3), triacylglycerol acyl hydrolase, is one of the most important enzymes in industrial enzyme preparations, which can catalyze the hydrolysis of natural substrate oil at the oil-water interface to generate fatty acids, glycerol and At the same time, lipase can also catalyze various chemical reactions such as transesterification, lipid transfer, lipid synthesis, alcoholysis, acidolysis, ammonolysis, etc., and has high catalytic efficiency and simple catalytic conditions. Therefore, it is widely used It is used in industries such as detergent, leather, oil processing, paper making, and daily chemicals.

[0003] A large amount of wastewater containing petroleum hydrocarbons will be produced in the process of petroleum production and pro...