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Method for preparing cigar tobacco through cellulase fermentation

A technology of cellulase and cigar tobacco leaves, which is applied in the fields of tobacco, application, and treatment of tobacco, to achieve the effects of improving bitterness, flammability and gray score, and obvious bitterness

Active Publication Date: 2015-09-02
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Improving the quality and use value of tobacco leaves

Method used

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  • Method for preparing cigar tobacco through cellulase fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The method for preparing cigar tobacco leaves by cellulase fermentation comprises the following steps:

[0024] 1) Alcoholize 30kg of the upper part of the cigar filler under natural conditions for one year, and then remoisturize the upper part under vacuum;

[0025] 2) Dissolving cellulase in deionized water to obtain a cellulase solution, wherein the cellulase activity in the cellulase solution is 600 U / ml;

[0026] 3) placing the leaf stalk of the tobacco leaves treated with moisture resurgence in step 1) in a cellulase solution and soaking the handle for 30 minutes, and then spraying the cellulase solution on the tobacco leaves at a weight ratio of 1:10 to obtain tobacco leaves containing cellulase, wherein , in every gram of tobacco leaves containing cellulase, the enzyme activity of cellulase is 55U; let stand for 24 hours to balance the moisture in the tobacco leaves, and then adjust the moisture content of the tobacco leaves to 25%;

[0027] 4) Fermenting the t...

Embodiment 2

[0030] The method for preparing cigar tobacco leaves by cellulase fermentation comprises the following steps:

[0031] 1) Aging 30kg of the middle part of the cigar filler under natural conditions for one year, and then re-moisture the upper part of the smoke under vacuum conditions;

[0032] 2) Dissolving cellulase in deionized water to obtain a cellulase solution, wherein the cellulase activity in the cellulase solution is 450 U / ml;

[0033] 3) placing the leaf stalk of the tobacco leaves treated with moisture resurgence in step 1) in a cellulase solution to soak the handle for 20 minutes, and then spraying the cellulase solution on the tobacco leaves at a weight ratio of 1:10 to obtain tobacco leaves containing cellulase, wherein , in every gram of tobacco leaves containing cellulase, the enzyme activity of cellulase is 45U; let stand for 24 hours to balance the moisture in the tobacco leaves, and then adjust the moisture content of the tobacco leaves to 25%;

[0034] 4) F...

Embodiment 3

[0037] The method for preparing cigar tobacco leaves by cellulase fermentation comprises the following steps:

[0038] 1) Aging 30kg of the lower part of the cigar filler under natural conditions for one year, and then re-moisture the upper part of the smoke under vacuum conditions;

[0039] 2) Dissolving cellulase in deionized water to obtain a cellulase solution, wherein the cellulase activity in the cellulase solution is 300 U / ml;

[0040] 3) placing the leaf stalk of the tobacco leaves treated with moisture resurgence in step 1) in a cellulase solution for 10 minutes, and then spraying the cellulase solution on the tobacco leaves at a weight ratio of 1:10 to obtain tobacco leaves containing cellulase, wherein , in every gram of tobacco leaves containing cellulase, the enzyme activity of cellulase is 30U; let stand for 24 hours to balance the moisture in the tobacco leaves, and then adjust the moisture content of the tobacco leaves to 24%;

[0041] 4) Fermenting the tobacc...

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Abstract

The invention discloses a method for preparing cigar tobacco through cellulase fermentation. The method comprises the steps that the petiole ends of the tobacco which is subjected to moisture regain are placed in a cellulase solution, the petioles are soaked for 10-30 min, the spray mist of the cellulase solution is sprayed on the tobacco based on the weight ratio of 1 to 5-15, the tobacco containing cellulase is obtained, standing is conducted until moisture balance is established, the tobacco with the moisture content of 25+ / -1% is fermented for 10-60 days under the conditions that the temperature range is 47-53 DEG C and the humidity range is 65-75%, and the cigar tobacco is obtained. According to the method for preparing the cigar tobacco through the cellulase fermentation, the cigar tobacco is taken as the object of study, the bitter taste of the cigar tobacco obtained through fermentation is obviously improved, the characteristics such as flavor, smoke, taste, combustibility and grayness are all improved, and the quality of the cigar tobacco is improved.

Description

technical field [0001] The invention relates to the field of cigar tobacco processing, in particular to a method for preparing cigar tobacco leaves by cellulase fermentation. Background technique [0002] Cellulose is the main structural component of tobacco leaves, which determines the integrity of tobacco leaves; cellulose has a good effect on the combustibility of tobacco leaves, and can increase the firepower of tobacco leaves. However, when the cellulose content in the tobacco leaves is too high, the tobacco leaf tissue is rough and easily broken; and the high cellulose content in the tobacco formula generally has a negative effect on the smoking quality, giving the smoke a sharp irritation and a "burning paper" smell , the smoke is hot and rough, and it will cause choking and coughing. The cellulose content in tobacco is generally about 11%, and it increases with the decrease of grade. [0003] Cellulase is a general term for a group of enzymes that hydrolyze cellulo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20A24B15/18
CPCA24B15/18A24B15/20
Inventor 胡捷张宁刘利平刘洋周斌
Owner HUBEI CHINA TOBACCO IND
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