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Immune protection application of staphylococcus aureus EsxA protein

A staphylococcus, immune protection technology, applied in the field of immune vaccines, can solve the problem of weakening pathogenicity and achieve remarkable results

Inactive Publication Date: 2015-09-02
THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The virulence factors produced by the secretion system of Staphylococcus aureus are important pathogenic factors, among which the VII secretion system is newly discovered, and EsxA is an ESAT-6-like virulence protein secreted by the VII secretion system. Studies have shown that the EsxA protein coding gene The deletion of the bacterium greatly weakened the pathogenicity of the

Method used

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  • Immune protection application of staphylococcus aureus EsxA protein
  • Immune protection application of staphylococcus aureus EsxA protein
  • Immune protection application of staphylococcus aureus EsxA protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1. Preparation of EsxA vaccine

[0014] The EsxA vaccines were prepared respectively at a mass ratio of EsxA protein / aluminum hydroxide adjuvant of 1:2.5.

[0015] 2. Immunization of BALB / c mice

[0016] Immunized mice:

[0017] EsxA vaccine group: On days 1, 7, and 21, 200 μL of vaccine containing 200 μg / mL EsxA and 500 μg / mL aluminum hydroxide adjuvant was subcutaneously injected into the back of each four-week-old mouse, and blood was collected intravenously on day 28 to detect anti- EsxA antibody production.

[0018] Control group: On days 1, 7, and 21, each mouse was subcutaneously injected with 200 μL of vaccine containing 500 μg / mL aluminum hydroxide adjuvant on the back, and blood was collected intravenously on day 28.

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Abstract

The invention discloses an immune protection application of staphylococcus aureus EsxA protein, and belongs to the technical field of an immune vaccine. The immune protection application of the staphylococcus aureus EsxA protein is achieved by preparing an EsxA vaccine capable of preventing and controlling staphylococcus aureus infection from the staphylococcus aureus EsxA protein. The preparation method of the EsxA vaccine comprises a step of blending EsxA protein and an aluminum hydroxide adjuvant at the mass ratio of 1: 2.5 so as to prepare the EsxA vaccine. The invention reveals the immune protection effect of the staphylococcus aureus EsxA protein for the first time; the protein can be used for preparing the EsxA vaccine which is capable of preventing and controlling staphylococcus aureus infection, and the obtained EsxA vaccine can achieve a significant effect on preventing and controlling the staphylococcus aureus infection.

Description

technical field [0001] The invention belongs to the technical field of immune vaccines, and in particular relates to the immune protection application of EsxA protein of Staphylococcus aureus. Background technique [0002] Staphylococcus aureus (Staphylococcus auresu, S.Aureus) is one of the most important pathogenic bacteria causing community and hospital infections, and there is no ideal vaccine to prevent and control the infectious diseases caused by this bacteria. The virulence factors produced by the secretion system of Staphylococcus aureus are important pathogenic factors, among which the VII secretion system is newly discovered, and EsxA is an ESAT-6-like virulence protein secreted by the VII secretion system. Studies have shown that the EsxA protein coding gene The deletion of the bacterium greatly weakened the virulence of the bacteria. Contents of the invention [0003] In order to overcome the shortcomings and deficiencies of the prior art, the primary purpose...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/085A61P31/04
Inventor 杜鸿张海方朱雪明王敏张萍
Owner THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV