Immune protection application of staphylococcus aureus EsxB protein
A staphylococcus, immune protection technology, applied in the field of immune vaccines, can solve the problem of weakening pathogenicity and achieve remarkable results
Inactive Publication Date: 2015-09-02
THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
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The virulence factors produced by the secretion system of Staphylococcus aureus are important pathogenic factors. Among them, the VII secretion system is newly discovered, and EsxB is an ESAT-6-like virulence protein secreted by the VII secretion system. Studies have shown that the EsxB protein coding gene The deletion of the bacterium greatly weakened the pathogenicity of the
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Embodiment 1
[0012] 1. Preparation of EsxB vaccine
[0013] The EsxB vaccine was prepared respectively with the mass ratio of EsxB protein / aluminum hydroxide adjuvant as 1:2.5.
[0014] 2. Immunization of BALB / c mice
[0015] Immunized mice:
[0016] EsxB vaccine group: on days 1, 7, and 21, 200 μL of vaccine containing 200 μg / mL EsxB and 500 μg / mL aluminum hydroxide adjuvant was subcutaneously injected into the back of each four-week-old mouse, and blood was collected intravenously on day 28 to detect anti- EsxB Antibody Production.
[0017] Control group: On days 1, 7, and 21, each mouse was subcutaneously injected with 200 μL of vaccine containing 500 μg / mL aluminum hydroxide adjuvant on the back, and blood was collected intravenously on day 28.
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The invention discloses an immune protection application of staphylococcus aureus EsxB protein, and belongs to the technical field of an immune vaccine. The immune protection application of the staphylococcus aureus EsxB protein is achieved by preparing an EsxB vaccine capable of preventing and controlling staphylococcus aureus infection from the staphylococcus aureus EsxB protein. The preparation method of the EsxB vaccine comprises a step of blending EsxB protein and an aluminum hydroxide adjuvant at the mass ratio of 1: 2.5 so as to prepare the EsxB vaccine. The invention reveals the immune protection effect of the staphylococcus aureus EsxB protein for the first time; the protein can be used for preparing the EsxB vaccine which is capable of preventing and controlling staphylococcus aureus infection, and the obtained EsxB vaccine can achieve a significant effect on preventing and controlling the staphylococcus aureus infection.
Description
technical field [0001] The invention belongs to the technical field of immune vaccines, and in particular relates to the immune protection application of Staphylococcus aureus EsxB protein. Background technique [0002] Staphylococcus aureus (S.Aureus) is one of the most important pathogenic bacteria causing community and hospital infections, and there is no ideal vaccine for the prevention and control of this bacterial infection. The virulence factors produced by the secretion system of Staphylococcus aureus are important pathogenic factors. Among them, the VII secretion system is newly discovered, and EsxB is an ESAT-6-like virulence protein secreted by the VII secretion system. Studies have shown that the EsxB protein coding gene The deletion of the bacterium greatly weakened the virulence of the bacteria. Contents of the invention [0003] In order to overcome the shortcomings and deficiencies of the prior art, the primary purpose of the present invention is to provid...
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IPC IPC(8): A61K39/085A61P31/04
Inventor 张海方杜鸿王敏周惠琴谢小芳
Owner THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV