Soil improvement microbial agent and preparation method thereof
A microbial agent and soil improvement technology, which is applied in the direction of fertilizer mixture, fertilization device, application, etc., can solve the problems of soil degradation, soil compaction, and damage to soil structure in cultivated land, and achieve the effect of soil improvement
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[0016] The preparation method of above-mentioned soil-improving type microbial bacterial agent, comprises the steps:
[0017] (1) Bacillus subtilis (Bacillus subtilis), Bacillus laterosporus (Bacillus laterosporus) and jelly-like Bacillus (Bacillus mucilaqinosus) were respectively expanded and cultured, and after pressure filtration and flash drying, they were mixed in proportion to prepare Obtain mixed microbial fermentation powder;
[0018] (2) Naturally aging and cleaning oyster shells, drying, calcining at 750-1250°C for 30-40 minutes, crushing and sieving to obtain oyster shell powder;
[0019] (3) The mixed microbial fermentation powder prepared in step (1), the oyster shell powder prepared in step (2), magnesium sulfate, zinc sulfate, and boric acid are uniformly mixed in proportion to prepare a soil-improving microbial bacterial agent.
[0020] Preferably according to the present invention, in described step (1), Bacillus subtilis (Bacillus subtilis), Bacillus lateros...
specific Embodiment approach
[0040] The technical solutions of the present invention will be further described below in conjunction with the examples, but the protection scope of the present invention is not limited thereto.
[0041] microbial source
[0042]Bacillus subtilis (Bacillus subtilis) comes from the China Agricultural Microorganism Culture Collection Management Center, and the strain number is ACCC 03120;
[0043] Bacillus laterosporus (Bacillus laterosporus) was obtained from the China Agricultural Microorganism Culture Collection and Management Center, and the strain number was ACCC 11079.
[0044] Bacillus mucilaqinosus was obtained from China Industrial Microorganism Culture Collection and Management Center, with the strain number CICC 21700;
[0045] culture medium
[0046] Solid slant medium for Bacillus subtilis and Bacillus laterosporus with the following components per liter:
[0047] Peptone 10g, beef powder 3.0g, sodium chloride 5.0g, agar 15.0g, add distilled water to 1000mL, adj...
Embodiment 1
[0053] A soil-improving microbial bacterial agent is characterized in that the components are as follows, all in parts by weight:
[0054] 4 parts of mixed microbial fermentation powder, 86 parts of oyster shell powder, 5 parts of magnesium sulfate, 2 parts of zinc sulfate, and 3 parts of boric acid;
[0055] The mixed microbial fermentation powder contains Bacillus subtilis (Bacillus subtilis), Bacillus laterosporus (Bacillus laterosporus) and jelly-like Bacillus (Bacillus mucilaqinosus) in a ratio of 1:1:3, and the soil improvement The effective number of live bacteria of the type microbial agent is 4.4×10 8 pcs / g.
[0056] The preparation method of above-mentioned soil-improving type microbial bacterial agent, comprises the steps:
[0057] (1) Bacillus subtilis (Bacillus subtilis), Bacillus laterosporus (Bacillus laterosporus) and jelly-like Bacillus (Bacillus mucilaqinosus) were expanded and cultured respectively, after pressure filtration, flash drying, and serial dilut...
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