Glucosamine-modified polyethyleneglycol-polylactic acid, preparation method therefor and application thereof
A technology of glucosamine and polyethylene glycol, applied in the field of glucosamine-modified polyethylene glycol-polylactic acid and its preparation, can solve the problems of high toxicity and side effects, lack of targeting, low curative effect, etc., and achieve high safety , improve the effect of tumor treatment
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Embodiment 1
[0031] A preparation method of glucosamine-modified polyethylene glycol-polylactic acid, comprising the following steps:
[0032] (1) Under the protection of nitrogen, 10g M-COOH-PEG 400 (Carboxy-modified polyethylene glycol, Beijing Jiankai Technology Co., Ltd.) was added to 80g of lactic acid (LA) solution, stirred at room temperature for 6hrs, vacuumed nitrogen replacement 3 times, heated and melted under vacuum (-0.1MPa) and Stir to mix the raw materials evenly, return to normal pressure under nitrogen protection, add the catalyst stannous octoate (2g) and react in a vacuum state for 25hrs; dissolve the product in dichloromethane, precipitate and purify with ether, and dry in vacuum under reduced pressure to obtain M-COOH- PEG 400 -PLA 720 polymer;
[0033] (2) Dissolve 5g of glucosamine (AG) in 10mL DMF at room temperature to form an AG solution; take 10g of M-COOH-PEG prepared in step (1) 400 -PLA 720 The polymer was dissolved in 20 mL of dimethylformamide (DMF) at ...
Embodiment 2
[0040] A preparation method of glucosamine-modified polyethylene glycol-polylactic acid, comprising the following steps:
[0041] (1) Under the protection of nitrogen, 50g M-COOH-PEG 2000(Carboxy-modified polyethylene glycol, Beijing Jiankai Technology Co., Ltd.) was added to 400g lactic acid (LA) solution, stirred at room temperature for 6hrs, vacuumed nitrogen replacement 3 times, heated and melted under vacuum (-0.1MPa) and Stir to mix the raw materials evenly, return to normal pressure under nitrogen protection, add the catalyst stannous octoate (10g) and react in a vacuum state for 30hrs; dissolve the product in dichloromethane, precipitate and purify with ether, and dry in vacuum under reduced pressure to obtain M-COOH- PEG 2000 -PLA 6600 polymer;
[0042] (2) Dissolve 20g of glucosamine (AG) in 40mL DMF at room temperature to form an AG solution; take 40g of M-COOH-PEG prepared in step (1) 2000 -PLA 6600 Dissolve the polymer in 80mL dimethylformamide (DMF) at room ...
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