A kind of equipment and process of edible fungus liquid fermentation primary strain cultivation and aseptic inoculation

Abstract

The invention provides an edible fungus liquid fermentation primary strain culture and sterile inoculation device which comprises a tank body and a fermentation tank, wherein the top of the tank body is provided with a feed port; the feed port is provided with a feed port cover; the top of the tank body is also provided with an air filter; the air filter is provided with a compressed air inlet pipe; a discharge pipeline is arranged in the tank body; one end of the discharge pipeline penetrates into the bottom of the tank body, and the other end protrudes out of the tank body; a stirrer is arranged in the fermentation tank; the top of the fermentation tank is provided with an inoculation pipeline; the upper wall of the inoculation pipeline is provided with a sterilizing steam inlet pipe; the lower wall of the inoculation pipeline is provided with a steam condensate outlet pipe; the front end of the discharge pipeline is provided with a discharge port; the orifice diameter of the discharge port is smaller than that of the inoculation pipeline; and the discharge port can form an abutting joint with the orifice of the inoculation pipeline. The device can directly form an abutting joint with the inoculation port of the fermentation tank; the primary strain is inoculated into the fermentation tank by utilizing pressure difference without external contact, so the probability of infectious microbe infection is reduced to almost zero in the inoculation process; and thus, the fermentation process can proceed normally, and the production efficiency is greatly enhanced.

Description

technical field [0001] The invention relates to equipment for the cultivation and aseptic inoculation of edible fungus liquid fermentation primary strains, and the inoculation process using the equipment, which belongs to the field of biological fermentation. Background technique [0002] In the existing liquid fermentation process of edible fungus strains, the first-class species is cultivated on a shaker with a triangular flask, and then the fermentation workshop utilizes an alcohol flame ring to open the inoculation port of the fermenter, and pour the first-class species in the triangular flask into the fermenter. In this process, the cleanliness of the fermentation workshop, the sterility of the inoculation port on the fermenter, the cleanliness of the triangular flask, and the proficiency of the operator all have a crucial impact on whether the inoculation is free of bacterial infection. Often the above A little carelessness of various factors in the inoculation proces...

AI Technical Summary

Problems solved by technology

In this process, the cleanliness of the fermentation workshop, the sterility of the inoculation port on the fermenter, the cleanliness of the triangular flask, and the proficiency of the operator all have a crucial impact on whether the inoculation is free of bacterial infection. Often the above A little carelessness of various factors in the inoculation process will cause the fermentation tank to be infected with miscellaneous bacteria, and in serious cases, the disaster of tank dumping will occur, affecting the entire production process and causing huge economic losses to the enterprise

Images