Manufacture technology of frost shiitake mushroom spawns

A production process and a technology for the frost mushroom, which are applied in the determination/inspection of fungi and microorganisms, microorganisms and other directions, can solve the problems of difficulty in large-scale cultivation and cultivation, low food value, and poor taste of the frost mushroom strain, so as to eliminate the The effect of infection with miscellaneous bacteria, short growth distance, and reduced possibility

Inactive Publication Date: 2017-12-19
西充星河生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a production process of velvet mushroom strains, which has the advantages of high inoculation efficiency and rapid germination, and solves the problems of poor taste, low edible value and difficulty in large-scale cultivation and planting of velvet velvet mushroom strains

Method used

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Examples

Experimental program
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Embodiment 1

[0048] This embodiment provides a kind of production process of velvet velvet mushroom spawn, comprising the following steps: test tube culture medium production, test tube seed transfer, test tube seed selection, secondary strain production, plate culture medium production, plate transfer, plate seed Selection, production of three-level strains, production of shake flask culture medium, shake flask seed inoculation, shake flask seed cultivation, shake flask seed selection, fermenter production, sterilization, empty barrel sampling, fermenter inoculation, fermenter seed cultivation and observation , Bacterial liquid sampling test, seeding, microscopic examination, inoculation pipeline sterilization, inoculation;

[0049] Wherein, the formula of the test tube medium used in the production of the test tube medium is: by weight, 190 parts of potatoes, 18 parts of glucose and agar powder, 9 parts of peptone, 1.3 parts of magnesium sulfate, and 2.8 parts of potassium dihydrogen phos...

Embodiment 2

[0198] This embodiment provides a kind of production process of velvet mushroom strain, test tube culture medium production, test tube seed transfer, test tube seed selection, secondary strain production, plate culture medium production, plate transfer, plate seed selection, three-stage Production of strains, production of shake flask medium, shake flask seed inoculation, shake flask seed cultivation, shake flask seed selection, fermenter production, sterilization, empty barrel sampling, fermenter inoculation, fermenter seed cultivation and observation, bacterial liquid sampling Detection, seeding, microscopic examination, sterilization of inoculation tubes, inoculation;

[0199] Among them, the formula of the test tube medium used in the production of the test tube medium is: by weight, 210 parts of potatoes, 22 parts of glucose and agar powder, 11 parts of peptone, 1.7 parts of magnesium sulfate, and 3.2 parts of potassium dihydrogen phosphate;

[0200] The formula of the pl...

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PUM

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Abstract

The invention discloses a production process of velvet mushroom strains, comprising the following steps: making test tube culture medium, transferring test tube species, selecting test tube species, making secondary strains, making plate culture medium, plate transfer, and plate species selection , Three-level strain production, shake flask culture medium preparation, shake flask seed inoculation, shake flask seed cultivation, shake flask seed selection, fermenter production, sterilization, empty barrel sampling, fermenter inoculation, fermenter seed cultivation and observation, Bacteria liquid sampling test, seeding, microscopic examination, inoculation pipeline sterilization, inoculation. The invention can improve the mycelium viability, prevent the infection of miscellaneous bacteria again after inoculation, increase the growth factors such as vitamin C, vitamin D, folic acid, etc., and effectively improve the efficiency of bacteria cultivation, and the peach wood powder with special fragrance can increase the taste of cream mushroom And flavor, improve its food value.

Description

technical field [0001] The invention relates to the technical field of strain production, in particular to a production process of mushroom strain. Background technique [0002] Edible mushrooms, also known as edible mushrooms, edible mushrooms, and edible fungi, refer to the general designation of large fungi with edible fruiting bodies. Edible mushrooms are widely distributed throughout the earth, and are most abundant in deciduous forests. Edible mushrooms are ideal natural or multifunctional foods. The most edible edible mushroom in the whole world so far, the scientific name is Agaricus bisporus, commonly known as mushroom. [0003] Cream mushrooms are rich in protein and amino acids, and their content is several to dozens of times that of ordinary vegetables and fruits. The protein content is 1.5-3.5%, which is 3 times that of Chinese cabbage, 6 times that of radish, and 17 times that of apple. The protein contained in 1 kg of dried mushrooms is equivalent to that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12Q1/04C12R1/645
CPCC12N1/14C12Q1/04
Inventor 甘远强卢莉黄宇王霞范建丽何琼珍
Owner 西充星河生物科技有限公司
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