A kind of method for solid culture of scarab metarhizium anisopliae

Abstract

The invention provides a metarhizium anisopliae solid culture method. The metarhizium anisopliae solid culture method comprises the following steps of: inoculating a metarhizium anisopliae liquid strain into a solid culture medium; placing the solid culture medium into a fermentation box and performing solid culture, wherein through holes are formed in the bottom surface of the fermentation box; at the early stage of solid culture and under the environment of 18-22 DEG C, stacking 4-8 layers of fermentation boxes with the solid culture mediums which inoculate the metarhizium anisopliae on an empty fermentation box without a through hole in the bottom surface layer by layer, covering a cover on the uppermost layer of the fermentation box and performing closed fermentation for 3-4 days; and at the later stage of solid culture, opening the cover of the uppermost layer of the fermentation box, separating all the fermentation boxes or stacking the fermentation boxes in a staggered mode, and performing open fermentation for 3-4 days under the environment that the temperature is 23-27 DEG C and the humidity is 80%. The metarhizium anisopliae solid culture method has the beneficial effects that humidity regulation and control are not needed basically in the fermentation process; the fermentation period is less than or equal to 8 days; the spore production level of the fermentation products is more than or equal to 1*10<10> spores per gram of fermentation products; the fermentation quality is stable; the pollution ratio is low; and scale production is easy to implement.

Description

technical field [0001] The invention belongs to the field of biological fermentation methods, and relates to a solid culture method for fungi, in particular to a solid culture method for Metarhizium anisopliae. Background technique [0002] Since the mid-20th century, due to the unlimited use of chemical pesticides, the environment and agricultural, forestry and animal husbandry products have been seriously polluted, endangering the health of humans and animals, and at the same time destroying the natural ecosystem. Biological control has been paid more and more attention by people, and the use of entomopathogenic microorganisms to control pests is one of the important means of biological control. Fungal insecticides have the characteristics of good diffusion effect, long persistence, unique body wall invasion, no resistance to pests and easy mass production, making fungal insecticides widely used in forest pests and soil pests. It has obvious advantages in the biological c...

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Problems solved by technology

[0002] Since the middle of the 20th century, due to the unlimited use of chemical pesticides, the environment and agricultural, forestry and animal husbandry products have been seriously polluted, endangering the health of humans and animals, and at the same time destroying the natural ecosystem

Combining the frame method with the plastic bag method can reduce the probability of pollution and the humidity requirements in the later stage of cultivation to a certain extent, but the humidity of mycelium growth in the early stage of cultivation cannot be guaranteed, and the solid material in the later stage of cultivation is not well ventilated, and the production efficiency is still low. , the product quality is unstable

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