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Rotary magnetic cell separation method

A magnetic separation and rotary technology, applied in the field of cell separation, can solve the problems of cell damage and long cell separation time, and achieve the effects of reducing damage, improving sorting efficiency and reducing requirements.

Inactive Publication Date: 2015-09-23
ARTHRO ANDA TIANJIN BIOLOGIC TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the magnetic separation racks produced by various companies are different in shape and magnetic field strength, their working methods rely on the static adsorption capacity of the magnetic field, resulting in a long cell separation time, generally 5-10 minutes or even longer
Moreover, the shorter the separation time, the higher the strength of the magnetic field used, which will cause greater damage to the cells.

Method used

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  • Rotary magnetic cell separation method

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Embodiment Construction

[0018] Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

[0019] Such as figure 1 Shown, among the figure 1 is ring magnet, and 2 is test tube.

[0020] The rotary cell magnetic separation method of the present invention comprises the following steps:

[0021] Step 1, put the sample: Put the turbid solution containing the target cells, labeled magnetic beads and stray cells into the test tube 2, and then insert the test tube into the middle of the ring magnet 1;

[0022] Step 2, rotating and separating: the test tube 2 is rotated in a certain way around the central axis of the ring magnet 1, and the rotation stops after a period of time;

[0023] Step 3, static separation: keep still for a period of time;

[0024] Step 4, cell collection: remove the supernatant.

[0025] In step 1, the magnetic field strength at the center of the ring magnet is 500-950mT.

[0026] Rotating in a certain way in the ...

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Abstract

The invention discloses a rotary magnetic cell separation method. The method includes the four steps of sample placing-in, rotary separation, static separation and cell collection. Magnetic beads are enabled to move in a direction away from axis under action of centrifugal force through a mode of enabling a test tube filled with cells and magnetic bead suspension liquid to rotate in an annular magnet, and distance from the magnetic beads to the annular magnet is shortened. The closer a position is from the annular magnet, the higher the strength of a magnetic field, and the larger the magnetic beads are adsorbed. The magnetic beads are stably adsorbed around the magnet through a stage of static separation. By the above mode, sorting time can be reduced greatly, sorting efficiency can be improved, and damage to the cells can be reduced effectively.

Description

technical field [0001] The invention relates to a cell separation method, in particular to a rotary magnetic cell separation method. Background technique [0002] Cell magnetic separation technology uses antibodies coupled to magnetic particles to identify and label target cell surface antigens with high specificity, and then uses the power of a magnetic field to screen magnetic beads to remove cells that are not bound to magnetic beads and obtain purified target cells. It is a common technique for cell sorting and enrichment. [0003] At present, the widely used magnetic cell separation product is the magnetic separation frame, which is a custom-made strong magnet. The cell suspension labeled with magnetic beads is attached to the surface of the magnet, and then stands still for a long time (5-10 minutes). , the cells bound to the magnetic beads will be adsorbed on the side wall of the magnet, and then the supernatant is removed by pouring or using a pipette, leaving the t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12N5/00
Inventor 刘晓华孙雪莲朱建民李召雨刘江周洪锐田建国叶青梅剑春黄慧玲李洲
Owner ARTHRO ANDA TIANJIN BIOLOGIC TECH
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