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Chicken FABP4 gene molecular genetic marker related to good chicken slaughtering character and application of chicken FABP4 gene molecular genetic marker

A technology of molecular genetic marker, FABP4, applied in the field of molecular biology

Inactive Publication Date: 2015-09-30
马韫韬
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching, there is no report on the application of FABP4 gene SNPs as genetic markers, especially as molecular genetic markers for good slaughtering traits of broiler chickens to screen or predict broiler chicken breeds with good slaughtering traits

Method used

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  • Chicken FABP4 gene molecular genetic marker related to good chicken slaughtering character and application of chicken FABP4 gene molecular genetic marker
  • Chicken FABP4 gene molecular genetic marker related to good chicken slaughtering character and application of chicken FABP4 gene molecular genetic marker
  • Chicken FABP4 gene molecular genetic marker related to good chicken slaughtering character and application of chicken FABP4 gene molecular genetic marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the cloning of different breed chicken FABP4 gene partial DNA sequence

[0026] 1. Collection and processing of chicken blood: randomly selected disease-free healthy chickens, including 670 Luqin No. 3 chickens and 60 Langya chickens, blood was collected from the underwing vein, and EDTA was used for anticoagulant treatment.

[0027]2. Genomic DNA extraction: DNA was extracted using Tiangen blood DNA extraction kit. Use 200 μL of fresh, frozen or blood with various anticoagulants, add 20 μL proteinase K solution, mix well; then add 200 μL buffer GB, mix well and place at 70°C for 10 minutes; add 200 μL absolute ethanol, shake and mix well At this time, flocculent precipitates will appear; transfer all the obtained solution and flocculent precipitates to the adsorption column CB3 in the collection tube, centrifuge at 12000rpm for 30 seconds and discard the waste liquid; add 500μL buffer GD, centrifuge at 12000rpm for 30 seconds and Discard the waste soluti...

Embodiment 2

[0037] Embodiment 2: Use TaqI enzyme to carry out specific enzyme digestion to the PCR product in embodiment 1

[0038] 1. Use TaqI enzyme to specifically digest the target gene. The action site of this type of specific enzyme is: 5'...T ↓ CGA…3’ or 3’…AGC ↑ T...5'.

[0039] 2. See Table 3 for the enzyme digestion reaction system.

[0040] Table 3 TaqI digestion reaction system

[0041]

[0042] After mixing the solutions of the reaction system according to the above system, 25 μL of paraffin oil was added to each reaction system to seal, and then placed in a constant temperature water bath at 65°C for 3-5 hours to allow the enzymatic cleavage reaction to fully proceed, followed by subsequent experiments.

[0043] 3. The digested products were analyzed by agarose gel electrophoresis. Then classify and summarize all samples according to different enzyme digestion results, and conclude different types.

[0044] There are three kinds of enzyme digestion results, namely c...

Embodiment 3

[0046] Example 3: Diagnostic application of molecular genetic markers prepared by the present invention in polymorphisms in different chicken populations

[0047] 1. Diagnosis in population polymorphism: 670 Luqin No. 3 chickens and 60 Langya chicken FABP4 genes were detected by specific enzyme digestion using the above-mentioned SNP polymorphism detection method.

[0048] 2. Frequency statistical analysis of SNP loci:

[0049] Genotype frequency refers to the ratio of the number of individuals of a certain genotype to the total number of individuals in a population. Paa=Aaa / N, where Paa represents the AA genotype frequency at a certain locus, Aaa represents the number of individuals with the AA genotype in the population, and N is the total number of the detection population.

[0050] Gene frequency refers to the relative ratio of the number of a certain gene to the total number of alleles in a population. The calculation formula can be written as: PA=(2NM+NA al +NA a2 +....

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Abstract

The invention discloses a chicken FABP4 gene molecular genetic marker related to a good meat chicken slaughtering character. The chicken FABP4 gene molecular genetic marker is a polymorphic site of the chicken FABP4 gene single nucleotide polymorphism sequence, is a basic group polymorphic site at the fifty-first site of a first exon of an FABP4 gene sequence being C or T, and is shown as the CC gene type or the CT gene type or the TT gene type, and the CC type is the good slaughtering character molecular genetic marker of the meat chicken. The invention further discloses the application of the mark in assisting in screening or predicting the meat chicken with the good slaughtering character. The experiment proves that relevance exists between the polymorphism of the special type digestion site and the production performance, multiple measurement indexes of the individual with the CC gene type are all higher than those of the individual with the TT gene type, and the individual with the CC gene type can be prompted to serve as a breeder chicken to breed the variety chicken with the good slaughtering character.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a chicken FABP4 gene molecular genetic marker related to excellent slaughter traits of broiler chickens and its application in assisting screening or prediction of broiler chicken breeds with excellent slaughter traits. Background technique [0002] Molecular genetic markers are based on DNA fragment length polymorphisms caused by species mutations, and have many advantages; (1) Directly detect differences in DNA levels without being limited by time and space; (2) The number of markers is abundant and the polymorphism is high ; (3) co-dominant markers, which can distinguish homozygotes and heterozygotes; (4) can explain the genetic variation of some individuals in the family; (5) can identify individuals of different sexes and ages. Single nucleotide polymorphism marker (single nucleotide polymorphism, SNP) is a type of DNA genetic marker, which is the pheno...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 马韫韬
Owner 马韫韬
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