A kind of differential protein in the serum of dairy cows in early pregnancy stage and its application

Abstract

The invention discloses differential protein in serum at the early pregnancy stage of a cow and application of the differential protein, and relates to the field of cow feeding. The differential protein includes pregnancy differential protein, non-pregnant differential protein and reverse differential protein. The pregnancy differential protein comprises ARHGAP42, EFEMP1, GTF2F1, LOXL2, CPB2, LYST, RDH11, TRABD, TRIM67 and / or TRMT112. The non-pregnant differential protein comprises IGL. The reverse differential protein comprises HBB and HP. The differential protein is used for judging whether the cow is pregnant or not and whether early embryo loss exists or not or increasing the embryo survival rate. By means of the differential protein, whether the fertilized cow is pregnant or not can be judged, synchronous estrus and the second time of artificial insemination can be conducted on the non-pregnant cow in time, early embryo loss early warning and progesterone preparation can be conducted, and the pregnancy rate and the breeding efficiency of cattle can be comprehensively increased.

Description

technical field [0001] The invention relates to the field of dairy cattle breeding, in particular to a differential protein in the serum of dairy cattle in the early pregnancy stage and use thereof. Background technique [0002] The reproductive performance of dairy cows not only affects the increase in the number and quality of dairy cows, but also affects the production performance and economic benefits of dairy cows. Therefore, the pregnancy rate of dairy cows is widely valued by the dairy industry in various countries. [0003] The current statistical results show that although the fertilization rate of dairy cows is 90%, the average calving rate is only 40% to 55%, and about 70% to 80% of embryo / fetal loss occurs in the first 3 weeks of gestation, of which 14 to 18 days. Early embryo loss during maternal pregnancy recognition predominates. [0004] Therefore, the pregnancy diagnosis of artificial insemination dairy cows should be carried out as soon as possible, and th...

AI Technical Summary

Problems solved by technology

[0006] The ELISA (enzyme-linked immunosorbent assay) kit is used to judge the content of PAG (pregnancy-associated glycoprotein) in the serum of dairy cows, and then judge whether the cows that have been fertilized for more than 28 days are pregnant. ) is pregnant, and it is difficult to check whether the cows 14-18 days after fertilization are empty-breasted cows

[0007] The invention patent application with the patent number 201410294733.0 provides a method and application for diagnosing whether a cow is pregnant based on BRCA2 protein and mRNA. This method judges whether a cow is pregnant according to the difference of BRCA2 protein in pregnant cows and empty cows. This method can only Early pregnancy diagnosis is carried out 18 days after artificial insemination of dairy cows, and since the expression trend of this protein in the early stages of pregnancy is not consistent, whether it can be used for early warning of early embryo loss in dairy cows is not yet defined

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