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A highly efficient anti-inflammatory pb ODN sequence and its application

A sequential, 0.034% KH2PO4 technology, applied in the direction of medical preparations containing active ingredients, antibacterial drugs, organic active ingredients, etc., to reduce costs, improve breeding efficiency, and reduce the amount of use

Active Publication Date: 2019-03-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, screening out characteristic sequences with obvious anti-inflammatory activity from probiotic DNA can not only provide more information for the development and utilization of probiotics, but also control inflammation after infection with various pathogens, which has considerable research prospects and value; However, there is no relevant literature on PBODN and reports on the prevention and treatment of porcine inflammation.

Method used

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  • A highly efficient anti-inflammatory pb ODN sequence and its application
  • A highly efficient anti-inflammatory pb ODN sequence and its application
  • A highly efficient anti-inflammatory pb ODN sequence and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The sequence of PB ODN (SEQ ID NO: 1): 5'-TCCAAGTACTTGGACTTGG-3', can be synthesized by existing genetic engineering or chemical methods, and the sequence has been treated with thiolation (thiolation treatment can refer to the conventional process carried out). After synthesis, a 10µg / ml preparation (PB ODN preparation) was prepared with sterile PBS solution and stored at -15°C for future use.

[0024] The PBS solution consists of: by weight percentage, 0.6% NaCl, 0.01% KCl, 0.0124% Na 2 HPO 4 , 0.014% KH 2 PO 4 Dissolve in water; then sterilize at 115°C for 20 minutes by damp heat autoclaving. Moist heat autoclaving is a commonly used sterilization method in this field, and its operation can be carried out with reference to the prior art.

[0025] Prevention: 0.1 ml of the PBODN preparation per kilogram of animal weight was subcutaneously injected one week before the pig's disease onset peak, and administered again 3 days later.

[0026] Treatment: Immediately af...

Embodiment 2

[0028] The sequence of PB ODN (SEQ ID NO: 1): 5'-TCCAAGTACTTGGACTTGG-3', can be synthesized by existing genetic engineering or chemical methods, and the sequence has been treated with thiolation (thiolation treatment can refer to the conventional process carried out). After synthesis, a 30 µg / ml preparation (PB ODN preparation) was prepared with sterile PBS solution and stored at -20°C for future use.

[0029] The PBS solution consists of: by weight percentage, 0.7% NaCl, 0.02% KCl, 0.0134% Na 2 HPO 4 , 0.024% KH 2 PO 4 Soluble in water; then sterilize at 117°C for 21 minutes by damp heat autoclaving. Moist heat autoclaving is a commonly used sterilization method in this field, and its operation can be carried out with reference to the prior art.

[0030] Prevention: 0.2 ml of the PBODN preparation per kilogram of animal weight was subcutaneously injected one week before the peak of disease incidence in pigs, and administered again 4 days later.

[0031] Treatment: Immed...

Embodiment 3

[0033] The sequence of PB ODN (SEQ ID NO: 1): 5'-TCCAAGTACTTGGACTTGG-3', can be synthesized by existing genetic engineering or chemical methods, and the sequence has been treated with thiolation (thiolation treatment can refer to the conventional process carried out). After synthesis, a 50µg / ml preparation (PB ODN preparation) was prepared with sterile PBS solution and stored at -22°C for future use.

[0034] The PBS solution consists of: by weight percentage, 0.8% NaCl, 0.03% KCl, 0.0144% Na 2 HPO 4 , 0.0245% KH 2 PO 4Soluble in water; then sterilize at 118°C for 22 minutes by damp heat autoclaving. Moist heat autoclaving is a commonly used sterilization method in this field, and its operation can be carried out with reference to the prior art.

[0035] Prevention: 0.3ml of the PBODN preparation was subcutaneously injected per kilogram of animal weight one week before the pig's disease peak, and administered again 5 days later.

[0036] Treatment: Immediately after the ...

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Abstract

The invention belongs to the technical field of biopharmacy, and particularly discloses an effectively anti-inflammatory PB ODN sequence and an application thereof. The sequence, shown as SEQ ID NO:1, is effectively anti-inflammatory; preparations containing the sequence are quite favorable for controlling swine diseases and inflammation and are capable of substituting for antibiotics totally or partly successfully so as to reduce usage of antibiotics and vaccinum; therefore, animal food is safer and meets requirement of environmental friendliness, cost of animal breeding production enterprises is reduced, and breeding efficiency of the animal breeding production enterprises can also be increased.

Description

technical field [0001] The present invention relates to the technical field of biopharmaceuticals, more specifically, to a high-efficiency anti-inflammatory PB ODN sequence and its application. Background technique [0002] Many domestic veterinary anti-inflammatory drugs mainly rely on antibiotics, but long-term use of antibiotics will lead to serious drug resistance. At present, many companies at home and abroad have begun to restrict the use of various antibiotics. The abuse of antibiotics has also greatly restricted my country's meat export of food products. Another way to prevent animal diseases is through the use of vaccines. However, there are many serotypes of various pathogenic bacteria, the vaccine cross-reaction, and the protective effect is poor. Therefore, there is an urgent need for a new type of safe anti-inflammatory agent. [0003] Probiotic DNA is the main effector of probiotics that can inhibit a variety of inflammatory reactions, and there are specific ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7105A61P31/04
Inventor 张玲华周洁安蔡海明马苗鹏
Owner SOUTH CHINA AGRI UNIV
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